CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011001
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Autophagy-related protein 21 
Protein Synonyms/Alias
 Cytoplasm to vacuole transport protein 21; Homologous with SVP1 protein 1; Maturation of proaminopeptidase I protein 1 
Gene Name
 ATG21 
Gene Synonyms/Alias
 CVT21; HSV1; MAI1; YPL100W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
222LLANATQKSVNGSNPubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Required for cytoplasm to vacuole transport (Cvt) vesicles formation and mitophagy. Involved in binding of phosphatidylethanolamine to ATG8 and in recruitment of ATG8 and ATG5 to the pre-autophagosomal structure. Protects ATG8 from ARG4- mediated cleavage. Essential for maturation of proaminopeptidase I. 
Sequence Annotation
 REPEAT 294 334 WD 1.
 REPEAT 346 385 WD 2.
 REPEAT 448 488 WD 3.
 MOTIF 342 346 FRRGT-motif.
 MOD_RES 213 213 Phosphothreonine.
 MOD_RES 237 237 Phosphoserine.  
Keyword
 Autophagy; Complete proteome; Cytoplasm; Phosphoprotein; Protein transport; Reference proteome; Repeat; Transport; Vacuole; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 496 AA 
Protein Sequence
MKVLQFNQDA TCCVVAASSH QISIFNCDPF GKCFEIDTKN SKKKTSNNNG SASNSESRNN 60
EESILITNGS RDRTDAEEEE DNEDNALVTG NILKEGEFVI EMLFSTSLIA IADRGQGLNK 120
GKKLKIVNTK RKCTICEIVF PHEIVDVVMN RKRMCVLLES DQIFIYDISC MKPLETIDLW 180
EDHYKRSQAN SFSNASNTGT LEGDSANLNR VATNLLANAT QKSVNGSNPS VRTRRNSLRS 240
KIRPRMVLSN DDRSILCFTA YSSPKKNKPN SEALYDVVIY DTLNVTPVNY LNSVHKGNVA 300
CLAVSHDGKL LATASDKGTI IRVFHTGVDS DYMSSRSLFK EFRRGTRLCN LYQLAFDKSM 360
TMIGCVGDTD TIHLFKLDDA SNSLPGDNSS NGHWNEEEYI LASNSNPSMG TPKEIPLSKP 420
RIANYFSKKI KSSIPNQNLS RNFAYITVNE SNRSCLGFPD EFPNQVYIAS DDGTFSIYSI 480
PSKPGECVLT KNNKFT 496 
Gene Ontology
 GO:0005829; C:cytosol; IDA:SGD.
 GO:0005768; C:endosome; IDA:SGD.
 GO:0000329; C:fungal-type vacuole membrane; IDA:SGD.
 GO:0070772; C:PAS complex; IBA:RefGenome.
 GO:0080025; F:phosphatidylinositol-3,5-bisphosphate binding; IBA:RefGenome.
 GO:0032258; P:CVT pathway; IMP:SGD.
 GO:0016236; P:macroautophagy; IMP:SGD.
 GO:0000422; P:mitochondrion degradation; IMP:SGD.
 GO:0034727; P:piecemeal microautophagy of nucleus; IMP:SGD.
 GO:0006497; P:protein lipidation; IMP:SGD.
 GO:0034497; P:protein localization to pre-autophagosomal structure; IMP:SGD.
 GO:0016050; P:vesicle organization; IMP:SGD. 
Interpro
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS