UniProt Accession

 EFTU_MOUSE; Q8BFR5; Q497E7; Q6P919 

Genbank Protein ID

 AK075857; AK084724; AK153135; AK152858; BC060959; BC100596 

Genbank Nucleotide ID

 BAC36008.1; BAC39263.1; BAE31747.1; BAE31551.1; AAH60959.1; AAI00597.1 

Protein Name

 Elongation factor Tu, mitochondrial 

Protein Synonyms/Alias

  

Gene Name

 Tufm 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
88	LAEGGGAKFKKYEEI	acetylation	[1]
88	LAEGGGAKFKKYEEI	ubiquitination	[2]
90	EGGGAKFKKYEEIDN	acetylation	[1]
91	GGGAKFKKYEEIDNA	acetylation	[1]
238	LGVKSVQKLLDAVDT	acetylation	[1]
256	VPTRDLDKPFLLPVE	acetylation	[1]
297	CELLGHNKNIRTVVT	acetylation	[1]
361	AQVYILSKEEGGRHK	acetylation	[1]

Reference

 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis (By similarity). 

Sequence Annotation

 NP_BIND 64 71 GTP (By similarity).
 NP_BIND 126 130 GTP (By similarity).
 NP_BIND 181 184 GTP (By similarity).
 MOD_RES 79 79 N6-acetyllysine (By similarity).
 MOD_RES 88 88 N6-acetyllysine (By similarity).
 MOD_RES 256 256 N6-acetyllysine (By similarity).
 MOD_RES 418 418 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; Elongation factor; GTP-binding; Mitochondrion; Nucleotide-binding; Protein biosynthesis; Reference proteome; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 452 AA 

Protein Sequence

Gene Ontology

 GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0003746; F:translation elongation factor activity; ISS:UniProtKB.
 GO:0006184; P:GTP catabolic process; IEA:GOC. 

Interpro

 IPR000795; EF_GTP-bd_dom.
 IPR027417; P-loop_NTPase.
 IPR009001; Transl_elong_EF1A/Init_IF2_C.
 IPR004161; Transl_elong_EFTu/EF1A_2.
 IPR004541; Transl_elong_EFTu/EF1A_bac/org.
 IPR004160; Transl_elong_EFTu/EF1A_C.
 IPR009000; Transl_elong_init/rib_B-barrel. 

Pfam

 PF00009; GTP_EFTU
 PF03144; GTP_EFTU_D2
 PF03143; GTP_EFTU_D3 

SMART

  

PROSITE

 PS00301; EFACTOR_GTP 

PRINTS

 PR00315; ELONGATNFCT.