CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004236
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Enoyl-CoA hydratase, mitochondrial 
Protein Synonyms/Alias
 Enoyl-CoA hydratase 1; Short-chain enoyl-CoA hydratase; SCEH 
Gene Name
 Echs1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
40FQYIITEKKGKNSSVacetylation[1]
101FAAGADIKEMQNRTFacetylation[1]
115FQDCYSGKFLSHWDHacetylation[1]
204RISAQDAKQAGLVSKacetylation[1]
288EKRKANFKDH*****acetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate. 
Sequence Annotation
 REGION 98 101 Substrate binding.
 BINDING 141 141 Substrate; via amide nitrogen.
 MOD_RES 101 101 N6-acetyllysine (By similarity).  
Keyword
 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Fatty acid metabolism; Lipid metabolism; Lyase; Mitochondrion; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 290 AA 
Protein Sequence
MAALRALLPR ACNSLLSPVR CPEFRRFASG ANFQYIITEK KGKNSSVGLI QLNRPKALNA 60
LCNGLIEELN QALETFEEDP AVGAIVLTGG EKAFAAGADI KEMQNRTFQD CYSGKFLSHW 120
DHITRIKKPV IAAVNGYALG GGCELAMMCD IIYAGEKAQF GQPEILLGTI PGAGGTQRLT 180
RAVGKSLAME MVLTGDRISA QDAKQAGLVS KIFPVETLVE EAIQCAEKIA NNSKIIVAMA 240
KESVNAAFEM TLTEGNKLEK KLFYSTFATD DRREGMSAFV EKRKANFKDH 290 
Gene Ontology
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:RGD.
 GO:0004300; F:enoyl-CoA hydratase activity; IDA:RGD.
 GO:0006635; P:fatty acid beta-oxidation; IMP:RGD. 
Interpro
 IPR001753; Crotonase_core_superfam.
 IPR018376; Enoyl-CoA_hyd/isom_CS. 
Pfam
 PF00378; ECH 
SMART
  
PROSITE
 PS00166; ENOYL_COA_HYDRATASE 
PRINTS