CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031287
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Methionine aminopeptidase 
Protein Synonyms/Alias
  
Gene Name
 METAP2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
58AGEQEPDKESGASVDubiquitination[1]
79ERSALEDKERDEDDEubiquitination[1]
98DGDGATGKKKKKKKKubiquitination[1, 2, 3]
186DCSRKLIKENGLNAGubiquitination[4]
249FTVTFNPKYDTLLKAubiquitination[4]
255PKYDTLLKAVKDATNubiquitination[4]
258DTLLKAVKDATNTGIubiquitination[1, 4, 5]
266DATNTGIKCAGIDVRubiquitination[2, 4, 5]
300DGKTYQVKPIRNLNGubiquitination[4]
319QYRIHAGKTVPIVKGubiquitination[4, 5]
325GKTVPIVKGGEATRMubiquitination[4, 5]
404LDRLGESKYLMALKNubiquitination[4, 6, 7]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
 Removes the N-terminal methionine from nascent proteins (By similarity). 
Sequence Annotation
  
Keyword
 Aminopeptidase; Cobalt; Complete proteome; Hydrolase; Metal-binding; Protease; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 455 AA 
Protein Sequence
MAGVEEVAAS GSHLNGDLDP DDREEGAAST AEEAAKKKRR KKKKSKGPSA AGEQEPDKES 60
GASVDEVARQ LERSALEDKE RDEDDEDGDG DGDGATGKKK KKKKKKRGRQ ECEYPPTQDG 120
RTAAWRTTSE EKKALDQASE EIWNDFREAA EAHRQVRKYV MSWIKPGMTM IEICEKLEDC 180
SRKLIKENGL NAGLAFPTGC SLNNCAAHYT PNAGDTTVLQ YDDICKIDFG THISGRIIDC 240
AFTVTFNPKY DTLLKAVKDA TNTGIKCAGI DVRLCDVGEA IQEVMESYEV EIDGKTYQVK 300
PIRNLNGHSI GQYRIHAGKT VPIVKGGEAT RMEEGEVYAI ETFGSTGKGV VHDDMECSHY 360
MKNFDVGHVP IRLPRTKHLL NVINENFGTL AFCRRWLDRL GESKYLMALK NLCDLGIVDP 420
YPPLCDIKGS YTAQFEHTIL LRPTCKEVVS RGDDY 455 
Gene Ontology
 GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008235; F:metalloexopeptidase activity; IEA:InterPro.
 GO:0009987; P:cellular process; IEA:InterPro.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 
Interpro
 IPR001714; Pept_M24_MAP.
 IPR000994; Pept_M24_structural-domain.
 IPR002468; Pept_M24A_MAP2.
 IPR018349; Pept_M24A_MAP2_BS.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF00557; Peptidase_M24 
SMART
  
PROSITE
 PS01202; MAP_2 
PRINTS
 PR00599; MAPEPTIDASE.