| Tag | Content |
|---|
| CPLM ID | CPLM-005361 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Bud site selection protein 5 |
Protein Synonyms/Alias | |
Gene Name | BUD5 |
Gene Synonyms/Alias | YCR038C; YCR38C; YCR526 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 571 | DGNDTFTKDGNNVNM | ubiquitination | [1] | | 637 | AYQVSIAKVPRLT** | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] |
Functional Description | GDP-GTP exchange factor (GEF) for the small GTPase BUD1/RSR1. Regulates the activity of BUD1 together with BUD2 which is a GTPase-activating protein (GAP) of BUD1. Required to produce both the axial and bipolar patterns of bud site selection. Determines the orientation of division axis. Overexpression can suppress mutations in PRP20 which is the GEF for GSP1. May be a cytoplasmic GEF for GSP1. Might also act on the Ras-like protein CDC42. Appears to bind to Ras proteins but not to activate them. |
Sequence Annotation | DOMAIN 224 339 N-terminal Ras-GEF.
DOMAIN 412 640 Ras-GEF. |
Keyword | Complete proteome; Cytoplasm; Guanine-nucleotide releasing factor; Reference proteome. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 642 AA |
Protein Sequence | MSPKNKYVYI CVEYIYIYFA KIHKQSTLSS DTTKMFVLID NVLAYLLEQD DLFVTARFAI 60
QGQIVSRRVN KIHISNITDV LLQQFISHTL PYNDNIVPKK ILDSMRTAVR QLLEATACVS 120
RECPLVKRSQ DIKRARKRLL SDWYRLGADA NMDAVLLVVN SAWRFLAVWR PFVNSIQHAT 180
QELYQNIAHY LLHGNVNIQR VTALIQLVMG QDDLLFSMDD VLQEVFRIQL YLNKMLPHNS 240
HKWQKPSPFD SANLLLNFRD WTTDNALLQE LLLSYPTINK NKHKNHSVPR LIQIWVESYW 300
QDSETTLKDI LNFWYSHLAE YYEYQELFAD IVQLFINKKR TRQLKIHYIG LTDKEIEENK 360
PPLDYENLFL QYEIDKTNAN DELCGATDLS DLLFQWKQGE LLEVEAFALN VSPWSLAKTL 420
TLLESSLYLD IETIEFTRHF KHNDTTIDSV FTLSNQLSSY VLETTLQQTH TISYWLQVAL 480
SCLYLRNLNS LASIITSLQN HSIERLSLPI DVKSDHLFQR LKVVVHPNNN YNVYRRTIKH 540
IFHSQLPCVP FTSLLIRDIT FIRDGNDTFT KDGNNVNMQK FNQITKIVAF AQYLQQKQYE 600
DIHCSNTTAR SLLGAMIKVH TLYNDNKDRA YQVSIAKVPR LT 642 |
Gene Ontology | GO:0005938; C:cell cortex; IEA:UniProtKB-SubCell. GO:0005935; C:cellular bud neck; IDA:SGD. GO:0000131; C:incipient cellular bud site; IDA:SGD. GO:0005088; F:Ras guanyl-nucleotide exchange factor activity; TAS:SGD. GO:0007120; P:axial cellular bud site selection; IMP:SGD. GO:0007121; P:bipolar cellular bud site selection; IMP:SGD. GO:0051056; P:regulation of small GTPase mediated signal transduction; IEA:InterPro. GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |