CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022863
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nuclear RNA export factor 1 
Protein Synonyms/Alias
 Tip-associated protein; Tip-associating protein; mRNA export factor TAP 
Gene Name
 NXF1 
Gene Synonyms/Alias
 TAP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
6**MADEGKSYSEHDDubiquitination[1]
117TSQDGTSKNWFKITIubiquitination[1]
121GTSKNWFKITIPYGRubiquitination[1]
173ASTASALKAVNYKILubiquitination[2, 3]
205HTILNELKPEQVEQLubiquitination[1]
230SQQALDLKGLRSDPDubiquitination[1, 2, 3, 4]
289DMSSIVQKAPNLKILubiquitination[1, 4]
294VQKAPNLKILNLSGNubiquitination[1, 4]
304NLSGNELKSERELDKubiquitination[1]
347AIRERFPKLLRLDGHubiquitination[1]
437SSLAEYFKDSRNVKKubiquitination[1]
609AFTHLKAKGEIPEVAubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5. 
Sequence Annotation
 DOMAIN 119 198 RRM.
 REPEAT 266 291 LRR 1.
 REPEAT 292 315 LRR 2.
 REPEAT 316 343 LRR 3.
 REPEAT 344 371 LRR 4.
 DOMAIN 386 536 NTF2.
 DOMAIN 565 619 TAP-C.
 REGION 2 371 Interaction with ALYREF/THOC4.
 MOTIF 67 100 Nuclear localization signal.
 MOTIF 83 110 Nuclear export signal.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 126 126 Nitrated tyrosine (By similarity).  
Keyword
 3D-structure; Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Host-virus interaction; Leucine-rich repeat; mRNA transport; Nitration; Nucleus; Reference proteome; Repeat; RNA-binding; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 619 AA 
Protein Sequence
MADEGKSYSE HDDERVNFPQ RKKKGRGPFR WKYGEGNRRS GRGGSGIRSS RLEEDDGDVA 60
MSDAQDGPRV RYNPYTTRPN RRGDTWHDRD RIHVTVRRDR APPERGGAGT SQDGTSKNWF 120
KITIPYGRKY DKAWLLSMIQ SKCSVPFTPI EFHYENTRAQ FFVEDASTAS ALKAVNYKIL 180
DRENRRISII INSSAPPHTI LNELKPEQVE QLKLIMSKRY DGSQQALDLK GLRSDPDLVA 240
QNIDVVLNRR SCMAATLRII EENIPELLSL NLSNNRLYRL DDMSSIVQKA PNLKILNLSG 300
NELKSERELD KIKGLKLEEL WLDGNSLCDT FRDQSTYISA IRERFPKLLR LDGHELPPPI 360
AFDVEAPTTL PPCKGSYFGT ENLKSLVLHF LQQYYAIYDS GDRQGLLDAY HDGACCSLSI 420
PFIPQNPARS SLAEYFKDSR NVKKLKDPTL RFRLLKHTRL NVVAFLNELP KTQHDVNSFV 480
VDISAQTSTL LCFSVNGVFK EVDGKSRDSL RAFTRTFIAV PASNSGLCIV NDELFVRNAS 540
SEEIQRAFAM PAPTPSSSPV PTLSPEQQEM LQAFSTQSGM NLEWSQKCLQ DNNWDYTRSA 600
QAFTHLKAKG EIPEVAFMK 619 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0042405; C:nuclear inclusion body; IEA:Compara.
 GO:0005643; C:nuclear pore; IEA:Compara.
 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0003729; F:mRNA binding; IEA:Compara.
 GO:0005487; F:nucleocytoplasmic transporter activity; IEA:Compara.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0010467; P:gene expression; TAS:Reactome.
 GO:0006406; P:mRNA export from nucleus; IDA:UniProtKB.
 GO:0016973; P:poly(A)+ mRNA export from nucleus; IEA:Compara.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 
Interpro
 IPR001611; Leu-rich_rpt.
 IPR002075; NTF2.
 IPR018222; Nuclear_transport_factor_2_euk.
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR005637; TAP_C_dom.
 IPR015245; Tap_RNA-bd.
 IPR003603; U2A'_phosphoprotein32A_C.
 IPR009060; UBA-like. 
Pfam
 PF00560; LRR_1
 PF02136; NTF2
 PF09162; Tap-RNA_bind
 PF03943; TAP_C 
SMART
 SM00446; LRRcap
 SM00804; TAP_C 
PROSITE
 PS51450; LRR
 PS50177; NTF2_DOMAIN
 PS50102; RRM
 PS51281; TAP_C 
PRINTS