CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010093
UniProt Accession
Genbank Protein ID
 U00089 
Genbank Nucleotide ID
Protein Name
 tRNA-specific 2-thiouridylase MnmA 
Protein Synonyms/Alias
  
Gene Name
 mnmA 
Gene Synonyms/Alias
 trmU; MPN_422; MP419 
Created Date
 July 27, 2013 
Organism
 Mycoplasma pneumoniae (strain ATCC 29342 / M129) 
NCBI Taxa ID
 272634 
Lysine Modification
Position
Peptide
Type
References
117MLCNRLIKFGLMRDYacetylation[1]
Reference
 [1] Cross-talk between phosphorylation and lysine acetylation in a genome-reduced bacterium.
 van Noort V, Seebacher J, Bader S, Mohammed S, Vonkova I, Betts MJ, Kühner S, Kumar R, Maier T, O'Flaherty M, Rybin V, Schmeisky A, Yus E, Stülke J, Serrano L, Russell RB, Heck AJ, Bork P, Gavin AC.
 Mol Syst Biol. 2012 Feb 28;8:571. [PMID: 22373819
Functional Description
 Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34 (By similarity). 
Sequence Annotation
 NP_BIND 9 16 ATP (By similarity).
 REGION 107 109 Interaction with target base in tRNA (By
 REGION 159 161 Interaction with tRNA (By similarity).
 ACT_SITE 112 112 Nucleophile (By similarity).
 ACT_SITE 209 209 Cysteine persulfide intermediate (By
 BINDING 35 35 ATP; via amide nitrogen and carbonyl
 BINDING 137 137 ATP; via amide nitrogen (By similarity).
 DISULFID 112 209 Alternate (By similarity).  
Keyword
 ATP-binding; Complete proteome; Cytoplasm; Disulfide bond; Nucleotide-binding; Reference proteome; RNA-binding; Transferase; tRNA processing; tRNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 370 AA 
Protein Sequence
MQNKTVFVGI SGGVDSAVSA LLLKQQYREV IGIFMECWDN TLNNDQLGHR AFNEHKSGCS 60
SKEDFREAQA IAQLLGIKLI KQNLVEPYWK QVFLPTIDAF KNGLTPNPDM LCNRLIKFGL 120
MRDYCKQLDP NSDFATGHYA ALSWDNNQPL LAIPKDKHKD QTYFLAHVKP AQLQDVVFPL 180
AHLLKTEVRQ IALAHHFSVA TKKDSTGICF IGERHFSDFL KNYLPVKPGV IYDWKTQRQL 240
GSHEGVWFYT TGQRSGLNLG GQAARNFVVE KDLKTNTLYV SSDPEDLQRR GITLSHFNWL 300
YQPNPLTQTV LVRIRHAQPL VQGHITVQPN NVVQVQLDQP IDRVTNGQYG VLYTQNGICL 360
GSGIITASQI 370 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016783; F:sulfurtransferase activity; IEA:InterPro.
 GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
 GO:0006400; P:tRNA modification; IEA:HAMAP. 
Interpro
 IPR023382; Adenine_a_hdrlase_dom.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR004506; tRNA-specific_2-thiouridylase. 
Pfam
 PF03054; tRNA_Me_trans 
SMART
  
PROSITE
  
PRINTS