CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015451
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Myosin phosphatase Rho-interacting protein 
Protein Synonyms/Alias
 M-RIP; Rho-interacting protein 3; RIP3; p116Rip 
Gene Name
 MPRIP 
Gene Synonyms/Alias
 KIAA0864; MRIP; RHOIP3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
5***MSAAKENPCRKFubiquitination[1]
396FKKGWLTKQYEDGQWubiquitination[1, 2, 3, 4]
1018NIRSKSLKEGLTVQEubiquitination[3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F- actin-binding region leads to disassembly of stress fibers in neuronal cells. 
Sequence Annotation
 DOMAIN 43 150 PH 1.
 DOMAIN 387 483 PH 2.
 REGION 1 383 Interaction with F-actin (By similarity).
 REGION 546 824 Interaction with RHOA.
 REGION 824 879 Interaction with PPP1R12A.
 MOD_RES 192 192 Phosphoserine.
 MOD_RES 220 220 Phosphoserine.
 MOD_RES 224 224 Phosphoserine.
 MOD_RES 226 226 Phosphoserine.
 MOD_RES 269 269 Phosphoserine.
 MOD_RES 289 289 Phosphoserine.
 MOD_RES 292 292 Phosphoserine.
 MOD_RES 295 295 Phosphothreonine.
 MOD_RES 320 320 Phosphothreonine (By similarity).
 MOD_RES 362 362 Phosphoserine.
 MOD_RES 365 365 Phosphoserine.
 MOD_RES 619 619 Phosphoserine.
 MOD_RES 891 891 Phosphoserine.
 MOD_RES 977 977 Phosphoserine.
 MOD_RES 993 993 Phosphoserine.
 MOD_RES 1014 1014 Phosphoserine (By similarity).
 MOD_RES 1016 1016 Phosphoserine.  
Keyword
 Actin-binding; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1025 AA 
Protein Sequence
MSAAKENPCR KFQANIFNKS KCQNCFKPRE SHLLNDEDLT QAKPIYGGWL LLAPDGTDFD 60
NPVHRSRKWQ RRFFILYEHG LLRYALDEMP TTLPQGTINM NQCTDVVDGE GRTGQKFSLC 120
ILTPEKEHFI RAETKEIVSG WLEMLMVYPR TNKQNQKKKR KVEPPTPQEP GPAKVAVTSS 180
SSSSSSSSSI PSAEKVPTTK STLWQEEMRT KDQPDGSSLS PAQSPSQSQP PAASSLREPG 240
LESKEEESAM SSDRMDCGRK VRVESGYFSL EKTKQDLKAE EQQLPPPLSP PSPSTPNHRR 300
SQVIEKFEAL DIEKAEHMET NAVGPSPSSD TRQGRSEKRA FPRKRDFTNE APPAPLPDAS 360
ASPLSPHRRA KSLDRRSTEP SVTPDLLNFK KGWLTKQYED GQWKKHWFVL ADQSLRYYRD 420
SVAEEAADLD GEIDLSACYD VTEYPVQRNY GFQIHTKEGE FTLSAMTSGI RRNWIQTIMK 480
HVHPTTAPDV TSSLPEEKNK SSCSFETCPR PTEKQEAELG EPDPEQKRSR ARERRREGRS 540
KTFDWAEFRP IQQALAQERV GGVGPADTHE PLRPEAEPGE LERERARRRE ERRKRFGMLD 600
ATDGPGTEDA ALRMEVDRSP GLPMSDLKTH NVHVEIEQRW HQVETTPLRE EKQVPIAPVH 660
LSSEDGGDRL STHELTSLLE KELEQSQKEA SDLLEQNRLL QDQLRVALGR EQSAREGYVL 720
QATCERGFAA MEETHQKKIE DLQRQHQREL EKLREEKDRL LAEETAATIS AIEAMKNAHR 780
EEMERELEKS QRSQISSVNS DVEALRRQYL EELQSVQREL EVLSEQYSQK CLENAHLAQA 840
LEAERQALRQ CQRENQELNA HNQELNNRLA AEITRLRTLL TGDGGGEATG SPLAQGKDAY 900
ELEVLLRVKE SEIQYLKQEI SSLKDELQTA LRDKKYASDK YKDIYTELSI AKAKADCDIS 960
RLKEQLKAAT EALGEKSPDS ATVSGYDIMK SKSNPDFLKK DRSCVTRQLR NIRSKSLKEG 1020
LTVQERLKLF ESRDLKKD 1038 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
 GO:0005543; F:phospholipid binding; IEA:InterPro. 
Interpro
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology. 
Pfam
 PF00169; PH 
SMART
 SM00233; PH 
PROSITE
 PS50003; PH_DOMAIN 
PRINTS