UniProt Accession

 6PGD_BACSU; P80859; P54546 

Genbank Protein ID

 D84432; AL009126 

Genbank Nucleotide ID

 BAA12615.1; CAB14318.2 

Protein Name

 6-phosphogluconate dehydrogenase, NADP(+)-dependent, decarboxylating 

Protein Synonyms/Alias

 GNTZII 

Gene Name

 gndA 

Gene Synonyms/Alias

 yqjI; BSU23860 

Created Date

 July 27, 2013 

Organism

 Bacillus subtilis (strain 168) 

NCBI Taxa ID

 224308 

Lysine Modification

Position	Peptide	Type	References
93	SLLPHLEKDDILIDG	acetylation	[1]
106	DGGNTYYKDTQRRNK	acetylation	[1]
309	LLSGPEVKPVTENKE	acetylation	[1]
459	HTYERTDKEGIFHTE	acetylation	[1]

Reference

 [1] The acetylproteome of Gram-positive model bacterium Bacillus subtilis.
 Kim D, Yu BJ, Kim JA, Lee YJ, Choi SG, Kang S, Pan JG.
 Proteomics. 2013 May;13(10-11):1726-36. [PMID: 23468065] 

Functional Description

 Catalyzes the oxidative decarboxylation of 6- phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH. Is the predominant 6-P- gluconate dehydrogenase isoenzyme in B.subtilis during growth on glucose and gluconate. 

Sequence Annotation

 NP_BIND 10 15 NADP (By similarity).
 NP_BIND 33 35 NADP (By similarity).
 NP_BIND 74 76 NADP (By similarity).
 REGION 128 130 Substrate binding (By similarity).
 REGION 185 186 Substrate binding (By similarity).
 ACT_SITE 182 182 Proton acceptor (By similarity).
 ACT_SITE 189 189 Proton donor (By similarity).
 BINDING 102 102 NADP (By similarity).
 BINDING 102 102 Substrate (By similarity).
 BINDING 190 190 Substrate (By similarity).
 BINDING 260 260 Substrate; via amide nitrogen (By
 BINDING 287 287 Substrate (By similarity).
 BINDING 446 446 Substrate; shared with dimeric partner
 BINDING 452 452 Substrate; shared with dimeric partner  

Keyword

 Complete proteome; Direct protein sequencing; Gluconate utilization; NADP; Oxidoreductase; Pentose shunt; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 469 AA 

Protein Sequence

Gene Ontology

 GO:0050661; F:NADP binding; IEA:InterPro.
 GO:0004616; F:phosphogluconate dehydrogenase (decarboxylating) activity; IEA:EC.
 GO:0019521; P:D-gluconate metabolic process; IEA:UniProtKB-KW.
 GO:0006098; P:pentose-phosphate shunt; IEA:UniProtKB-UniPathway. 

Interpro

 IPR008927; 6-PGluconate_DH_C-like.
 IPR006114; 6PGDH_C.
 IPR006113; 6PGDH_decarbox.
 IPR006115; 6PGDH_NADP-bd.
 IPR006184; 6PGdom_BS.
 IPR013328; DH_multihelical.
 IPR012284; Fibritin/6PGD_C-extension.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF00393; 6PGD
 PF03446; NAD_binding_2 

SMART

  

PROSITE

 PS00461; 6PGD 

PRINTS