UniProt Accession

 DHB12_RAT; Q6P7R8; Q9Z1B9 

Genbank Protein ID

 U81186; BC061543 

Genbank Nucleotide ID

 AAD00504.1; AAH61543.1 

Protein Name

 Estradiol 17-beta-dehydrogenase 12 

Protein Synonyms/Alias

 17-beta-hydroxysteroid dehydrogenase 12; 17-beta-HSD 12; 3-ketoacyl-CoA reductase; KAR 

Gene Name

 Hsd17b12 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
155	PDLDNTIKKLININV	methylation	[1]
156	DLDNTIKKLININVL	methylation	[1]
299	KTIMGFNKSLRNRYL	acetylation	[2]

Reference

 [1] A method for the comprehensive proteomic analysis of membrane proteins.
 Wu CC, MacCoss MJ, Howell KE, Yates JR 3rd.
 Nat Biotechnol. 2003 May;21(5):532-8. [PMID: 12692561]
 [2] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Catalyzes the transformation of estrone (E1) into estradiol (E2), suggesting a central role in estrogen formation. Its strong expression in ovary and mammary gland suggest that it may constitute the major enzyme responsible for the conversion of E1 to E2 in females. Also has 3-ketoacyl-CoA reductase activity, reducing both long chain 3-ketoacyl-CoAs and long chain fatty acyl-CoAs, suggesting a role in long fatty acid elongation (By similarity). 

Sequence Annotation

 NP_BIND 50 79 NADP (By similarity).
 MOTIF 308 312 Di-lysine motif (By similarity).
 ACT_SITE 202 202 Proton acceptor (By similarity).
 BINDING 189 189 Substrate (By similarity).  

Keyword

 Complete proteome; Endoplasmic reticulum; Lipid biosynthesis; Lipid metabolism; Membrane; NADP; Oxidoreductase; Reference proteome; Steroid biosynthesis; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 312 AA 

Protein Sequence

Gene Ontology

 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0031012; C:extracellular matrix; IEA:Compara.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0004303; F:estradiol 17-beta-dehydrogenase activity; IEA:EC.
 GO:0008201; F:heparin binding; IEA:Compara.
 GO:0006703; P:estrogen biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0030198; P:extracellular matrix organization; IEA:Compara.
 GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0010811; P:positive regulation of cell-substrate adhesion; IEA:Compara. 

Interpro

 IPR002198; DH_sc/Rdtase_SDR.
 IPR002347; Glc/ribitol_DH.
 IPR016040; NAD(P)-bd_dom.
 IPR020904; Sc_DH/Rdtase_CS. 

Pfam

 PF00106; adh_short 

SMART

  

PROSITE

 PS00061; ADH_SHORT 

PRINTS

 PR00081; GDHRDH.
 PR00080; SDRFAMILY.