UniProt Accession

 SGF73_YEAST; P53165; D6VU75 

Genbank Protein ID

 Z72588; Z72587; BK006941 

Genbank Nucleotide ID

 CAA96770.1; CAA96769.1; DAA08036.1 

Protein Name

 SAGA-associated factor 73 

Protein Synonyms/Alias

 73 kDa SAGA-associated factor; SAGA histone acetyltransferase complex 73 kDa subunit 

Gene Name

 SGF73 

Gene Synonyms/Alias

 YGL066W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
12	DAEIKGIKPKVIEEY	ubiquitination	[1]
33	GPSNDSWKSLMSSAK	acetylation	[2]
40	KSLMSSAKDTPLQYD	acetylation	[2]
171	SNGANYKKNDSSFNP	acetylation	[3]
180	DSSFNPLKRSTSMES	acetylation	[2, 4]
199	NMDTKRSKTGTPQTF	acetylation	[2, 3, 4]
211	QTFSSSIKKQKKVKQ	acetylation	[2, 3, 4]
212	TFSSSIKKQKKVKQR	acetylation	[4]
224	KQRNPTEKHLIDFNK	acetylation	[2, 3]
281	YHREHQTKIGAAAEK	acetylation	[2]
288	KIGAAAEKRAKQQEL	acetylation	[2, 3]
300	QELQKLQKQIQKEQK	acetylation	[3]
555	QQQLQQQKFEAAASY	acetylation	[2]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [3] In-depth profiling of post-translational modifications on the related transcription factor complexes TFIID and SAGA.
 Mischerikow N, Spedale G, Altelaar AF, Timmers HT, Pijnappel WW, Heck AJ.
 J Proteome Res. 2009 Nov;8(11):5020-30. [PMID: 19731963]
 [4] mChIP-KAT-MS, a method to map protein interactions and acetylation sites for lysine acetyltransferases.
 Mitchell L, Huard S, Cotrut M, Pourhanifeh-Lemeri R, Steunou AL, Hamza A, Lambert JP, Zhou H, Ning Z, Basu A, Côté J, Figeys DA, Baetz K.
 Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1641-50. [PMID: 23572591] 

Functional Description

 Functions as component of the transcription regulatory histone acetylation (HAT) complex SAGA. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). 

Sequence Annotation

 DOMAIN 220 286 SCA7.  

Keyword

 3D-structure; Complete proteome; Nucleus; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 657 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:SGD.
 GO:0071819; C:DUBm complex; IDA:SGD.
 GO:0000124; C:SAGA complex; IDA:SGD.
 GO:0046695; C:SLIK (SAGA-like) complex; IPI:SGD.
 GO:0008047; F:enzyme activator activity; IDA:SGD.
 GO:0005198; F:structural molecule activity; IMP:SGD.
 GO:0016573; P:histone acetylation; IMP:SGD.
 GO:0016578; P:histone deubiquitination; IMP:SGD.
 GO:0006406; P:mRNA export from nucleus; IMP:SGD.
 GO:0045899; P:positive regulation of RNA polymerase II transcriptional preinitiation complex assembly; IMP:SGD.
 GO:0006461; P:protein complex assembly; IMP:SGD.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR013243; SCA7_dom. 

Pfam

 PF08313; SCA7 

SMART

  

PROSITE

 PS51505; SCA7 

PRINTS