CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014649
UniProt Accession
Genbank Protein ID
 U50194 
Genbank Nucleotide ID
Protein Name
 Tripeptidyl-peptidase 2 
Protein Synonyms/Alias
 TPP-2; Tripeptidyl aminopeptidase; Tripeptidyl-peptidase II; TPP-II 
Gene Name
 Tpp2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
401AEYSLREKLPANQYTubiquitination[1]
655AFTDVHFKPGQIRRHacetylation[2]
655AFTDVHFKPGQIRRHubiquitination[1]
880YPHQYSLKLEKGDYTacetylation[2]
883QYSLKLEKGDYTIRLacetylation[2]
904ISDLDRLKDLPFIVSacetylation[2]
958VTSLPDDKIPKGAGPacetylation[2]
958VTSLPDDKIPKGAGPubiquitination[1]
1110RPDAATIKNDMDKQKacetylation[2]
1200KMYGRGLKFATKLVEacetylation[2]
1204RGLKFATKLVEEKPTacetylation[2]
1209ATKLVEEKPTKENWKacetylation[2]
Reference
 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113]
 [2] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Component of the proteolytic cascade acting downstream of the 26S proteasome in the ubiquitin-proteasome pathway. May be able to complement the 26S proteasome function to some extent under conditions in which the latter is inhibited (By similarity). 
Sequence Annotation
 ACT_SITE 44 44 Charge relay system (By similarity).
 ACT_SITE 264 264 Charge relay system (By similarity).
 ACT_SITE 449 449 Charge relay system (By similarity).
 MOD_RES 2 2 N-acetylalanine (By similarity).
 CROSSLNK 1005 1005 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 1013 1013 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Acetylation; Aminopeptidase; Complete proteome; Cytoplasm; Hydrolase; Isopeptide bond; Nucleus; Protease; Reference proteome; Serine protease; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1249 AA 
Protein Sequence
MATAATEEPF PFHGLLPKKE TGASSFLCRY PEYDGRGVLI AVLDTGVDPG APGMQVTTDG 60
KPKIIDIIDT TGSGDVNTAT EVEPKDGEIT GLSGRVLKIP ANWTNPSGKY HIGIKNGYDF 120
YPKALKERIQ KERKEKIWDP IHRVALAEAC RKQEEFDIAN NGSSQANKLI KEELQSQVEL 180
LNSFEKKYSD PGPVYDCLVW HDGETWRACV DSNENGDLGK STVLRNYKEA QEYGSFGTAE 240
MLNYSVNIYD DGNLLSIVTS GGAHGTHVAS IAAGHFPEEP ERNGVAPGAQ ILSIKIGDTR 300
LSTMETGTGL IRAMIEVINH KCDLVNYSYG EATHWPNSGR ICEVINEAVW KHNTIYVSSA 360
GNNGPCLSTV GCPGGTTSSV IGVGAYVSPD MMVAEYSLRE KLPANQYTWS SRGPSADGAL 420
GVSISAPGGA IASVPNWTLR GTQLMNGTSM SSPNACGGIA LVLSGLKANN VDYTVHSVRR 480
ALENTAIKAD NIEVFAQGHG IIQVDKAYDY LIQNTSFANR LGFTVTVGNN RGIYLRDPVQ 540
VAAPSDHGVG IEPVFPENTE NSEKISFQLH LALTSNSSWV QCPSHLELMN QCRHINIRVD 600
PRGLREGLHY TEVCGYDIAS PNAGPLFRVP ITAVIAAKVN ESSHYDLAFT DVHFKPGQIR 660
RHFVEVPEGA TWAEVTVCSC SSEVSAKFVL HAVQLVKQRA YRSHEFYKFC SLPEKGTLIE 720
AFPVLGGKAI EFCIARWWAS LSDVNIDYTI SFHGIVCTAP QLNIHASEGI NRFDVQSSLK 780
YEDLAPCITL KSWVQTLRPV NAKTRPLGSR DVLPNNRQLY EMVLTYSFHQ PKSGEVTPSC 840
PLLCELLYES EFDSQLWIIF DQNKRQMGSG DAYPHQYSLK LEKGDYTIRL QIRHEQISDL 900
DRLKDLPFIV SHRLSNTLSL DIHENHSLAL LGKKKSSSLT LPPKYNQPFF VTSLPDDKIP 960
KGAGPGCYLA GSLTLSKTEL GKKADVIPVH YYLIPPPTKT KNGSKDKEKD SEKEKDLKEE 1020
FTEALRDLKI QWMTKLDSTD IYNELKETYP AYLPLYVARL HQLDAEKERM KRLNEIVDAA 1080
NAVISHIDQT ALAVYIAMKT DPRPDAATIK NDMDKQKSTL VDALCRKGCA LADHLLHAQP 1140
HDGAAAGDAE AKEEEGESTL ESLSETYWET TKWTDLFDTK VLTFAYKHAL VNKMYGRGLK 1200
FATKLVEEKP TKENWKNCIQ LMKLLGWTHC ASFTENWLPI MYPPDYCVF 1249 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-KW.
 GO:0042277; F:peptide binding; IDA:RGD.
 GO:0004252; F:serine-type endopeptidase activity; IBA:RefGenome.
 GO:0008240; F:tripeptidyl-peptidase activity; IDA:RGD.
 GO:0006508; P:proteolysis; IDA:RGD. 
Interpro
 IPR000209; Peptidase_S8/S53_dom.
 IPR022398; Peptidase_S8_His-AS.
 IPR023828; Peptidase_S8_Ser-AS.
 IPR015500; Peptidase_S8_subtilisin-rel.
 IPR022229; Peptidase_S8A_TPPII. 
Pfam
 PF00082; Peptidase_S8
 PF12580; TPPII 
SMART
  
PROSITE
 PS00136; SUBTILASE_ASP
 PS00137; SUBTILASE_HIS
 PS00138; SUBTILASE_SER 
PRINTS
 PR00723; SUBTILISIN.