CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011905
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transducin beta-like protein 3 
Protein Synonyms/Alias
 WD repeat-containing protein SAZD 
Gene Name
 TBL3 
Gene Synonyms/Alias
 SAZD 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
12AAGVGRFKTNYAVERubiquitination[1]
20TNYAVERKIEPFYKGubiquitination[1]
26RKIEPFYKGGKAQLDacetylation[2]
26RKIEPFYKGGKAQLDubiquitination[1]
29EPFYKGGKAQLDQTGubiquitination[1, 3, 4]
105GSVTRLWKAIHTAPVubiquitination[1]
328ARSLRLQKQFAGYSEubiquitination[1, 5, 6, 7, 8]
407VRIWRMNKAGQVMCVubiquitination[3]
433TVCCSRLKESFLVTGubiquitination[1]
453VKLWPLPKALLSKNTubiquitination[5, 8]
458LPKALLSKNTAPDNGubiquitination[1, 6, 7]
492VAIAPNDKLLATGSQubiquitination[1, 6, 7]
504GSQDRTAKLWALPQCubiquitination[1]
588SGSDGLVKLWTIKNNubiquitination[1, 7]
593LVKLWTIKNNECVRTubiquitination[1]
607TLDAHEDKVWGLHCSubiquitination[1]
633DSRVILWKDVTEAEQubiquitination[1, 6, 7]
664LDNLLHEKRYLRALGubiquitination[1, 6]
698RDPEACEKLEATMLRubiquitination[1]
711LRLRRDQKEALLRFCubiquitination[6]
804PTPWETHKGALP***ubiquitination[1, 6, 7]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
  
Sequence Annotation
 REPEAT 64 105 WD 1.
 REPEAT 107 146 WD 2.
 REPEAT 149 190 WD 3.
 REPEAT 193 232 WD 4.
 REPEAT 245 284 WD 5.
 REPEAT 290 329 WD 6.
 REPEAT 332 372 WD 7.
 REPEAT 374 413 WD 8.
 REPEAT 419 459 WD 9.
 REPEAT 477 516 WD 10.
 REPEAT 519 560 WD 11.
 REPEAT 562 602 WD 12.
 REPEAT 604 642 WD 13.  
Keyword
 Complete proteome; Nucleus; Polymorphism; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 808 AA 
Protein Sequence
MAETAAGVGR FKTNYAVERK IEPFYKGGKA QLDQTGQHLF CVCGTRVNIL EVASGAVLRS 60
LEQEDQEDIT AFDLSPDNEV LVTASRALLL AQWAWQEGSV TRLWKAIHTA PVATMAFDPT 120
STLLATGGCD GAVRVWDIVR HYGTHHFRGS PGVVHLVAFH PDPTRLLLFS SATDAAIRVW 180
SLQDRSCLAV LTAHYSAVTS LAFSADGHTM LSSGRDKICI IWDLQSCQAT RTVPVFESVE 240
AAVLLPEEPV SQLGVKSPGL YFLTAGDQGT LRVWEAASGQ CVYTQAQPPG PGQELTHCTL 300
AHTAGVVLTA TADHNLLLYE ARSLRLQKQF AGYSEEVLDV RFLGPEDSHV VVASNSPCLK 360
VFELQTSACQ ILHGHTDIVL ALDVFRKGWL FASCAKDQSV RIWRMNKAGQ VMCVAQGSGH 420
THSVGTVCCS RLKESFLVTG SQDCTVKLWP LPKALLSKNT APDNGPILLQ AQTTQRCHDK 480
DINSVAIAPN DKLLATGSQD RTAKLWALPQ CQLLGVFSGH RRGLWCVQFS PMDQVLATAS 540
ADGTIKLWAL QDFSCLKTFE GHDASVLKVA FVSRGTQLLS SGSDGLVKLW TIKNNECVRT 600
LDAHEDKVWG LHCSRLDDHA LTGASDSRVI LWKDVTEAEQ AEEQARQEEQ VVRQQELDNL 660
LHEKRYLRAL GLAISLDRPH TVLTVIQAIR RDPEACEKLE ATMLRLRRDQ KEALLRFCVT 720
WNTNSRHCHE AQAVLGVLLR REAPEELLAY EGVRAALEAL LPYTERHFQR LSRTLQAAAF 780
LDFLWHNMKL PVPAAAPTPW ETHKGALP 808 
Gene Ontology
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0032040; C:small-subunit processome; IEA:InterPro.
 GO:0005057; F:receptor signaling protein activity; TAS:ProtInc.
 GO:0007199; P:G-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger; TAS:ProtInc.
 GO:0006364; P:rRNA processing; IEA:InterPro. 
Interpro
 IPR020472; G-protein_beta_WD-40_rep.
 IPR013934; SSU_processome_Utp13.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF08625; Utp13
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00320; GPROTEINBRPT.