UniProt Accession

 ADK_MOUSE; P55264; Q6JAM3; Q91VJ3 

Genbank Protein ID

 AY540996; AY540997; BC009659; U26589 

Genbank Nucleotide ID

 AAT07065.1; AAT07066.1; AAH09659.1; AAA91649.1 

Protein Name

 Adenosine kinase 

Protein Synonyms/Alias

 AK; Adenosine 5'-phosphotransferase 

Gene Name

 Adk 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
46	VDKDFLDKYSLKPND	ubiquitination	[1]
50	FLDKYSLKPNDQILA	acetylation	[2, 3]
50	FLDKYSLKPNDQILA	ubiquitination	[1]
60	DQILAEDKHKELFDE	ubiquitination	[1]
62	ILAEDKHKELFDELV	ubiquitination	[1]
70	ELFDELVKKFKVEYH	acetylation	[4]
70	ELFDELVKKFKVEYH	ubiquitination	[1]
73	DELVKKFKVEYHAGG	acetylation	[5]
73	DELVKKFKVEYHAGG	ubiquitination	[1]
110	FGCIGIDKFGEILKR	acetylation	[5]
110	FGCIGIDKFGEILKR	ubiquitination	[1]
116	DKFGEILKRKAADAH	acetylation	[5]
116	DKFGEILKRKAADAH	ubiquitination	[1]
161	LAAANCYKKEKHLDL	acetylation	[2]
161	LAAANCYKKEKHLDL	ubiquitination	[1]
164	ANCYKKEKHLDLERN	acetylation	[2]
164	ANCYKKEKHLDLERN	ubiquitination	[1]
177	RNWVLVEKARVYYIA	acetylation	[5]
255	REQGFETKDIKEIAK	acetylation	[2, 3, 6, 7]
255	REQGFETKDIKEIAK	ubiquitination	[1]
357	TGCTFPEKPDFH***	acetylation	[2, 5]
357	TGCTFPEKPDFH***	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [6] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [7] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599] 

Functional Description

 ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides. 

Sequence Annotation

 ACT_SITE 316 316 By similarity.
 METAL 147 147 Magnesium 2 (By similarity).
 MOD_RES 76 76 Phosphotyrosine (By similarity).  

Keyword

 Alternative splicing; ATP-binding; Complete proteome; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein; Purine salvage; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 361 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:MGI.
 GO:0005634; C:nucleus; IDA:MGI.
 GO:0004001; F:adenosine kinase activity; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0016773; F:phosphotransferase activity, alcohol group as acceptor; IEA:InterPro.
 GO:0046085; P:adenosine metabolic process; IEA:Compara.
 GO:0044209; P:AMP salvage; IEA:UniProtKB-UniPathway.
 GO:0032922; P:circadian regulation of gene expression; IEA:Compara.
 GO:0006175; P:dATP biosynthetic process; IMP:MGI.
 GO:0010613; P:positive regulation of cardiac muscle hypertrophy; IEA:Compara.
 GO:0042102; P:positive regulation of T cell proliferation; IEA:Compara.
 GO:0006166; P:purine ribonucleoside salvage; IDA:MGI.
 GO:0044342; P:type B pancreatic cell proliferation; IEA:Compara. 

Interpro

 IPR001805; Adenokinase.
 IPR002173; Carboh/pur_kinase_PfkB_CS.
 IPR011611; PfkB_dom. 

Pfam

 PF00294; PfkB 

SMART

  

PROSITE

 PS00583; PFKB_KINASES_1
 PS00584; PFKB_KINASES_2 

PRINTS

 PR00989; ADENOKINASE.