CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014114
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 BRO1 domain-containing protein BROX 
Protein Synonyms/Alias
 BRO1 domain- and CAAX motif-containing protein 
Gene Name
 BROX 
Gene Synonyms/Alias
 BROFTI; C1orf58 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
148KEVHRSLKIAAGIFKubiquitination[1]
155KIAAGIFKHLKESHLubiquitination[2, 3]
158AGIFKHLKESHLPKLubiquitination[1]
283EAEKLYAKAEALCKEacetylation[4]
334NGFIYFQKIPTEAPQubiquitination[2, 3, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965
Functional Description
  
Sequence Annotation
 DOMAIN 90 408 BRO1.
 MOD_RES 283 283 N6-acetyllysine.
 MOD_RES 408 408 Cysteine methyl ester (Probable).
 LIPID 408 408 S-farnesyl cysteine.  
Keyword
 3D-structure; Acetylation; Complete proteome; Lipoprotein; Membrane; Methylation; Prenylation; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 411 AA 
Protein Sequence
MTHWFHRNPL KATAPVSFNY YGVVTGPSAS KICNDLRSSR ARLLELFTDL SCNPEMMKNA 60
ADSYFSLLQG FINSLDESTQ ESKLRYIQNF KWTDTLQGQV PSAQQDAVFE LISMGFNVAL 120
WYTKYASRLA GKENITEDEA KEVHRSLKIA AGIFKHLKES HLPKLITPAE KGRDLESRLI 180
EAYVIQCQAE AQEVTIARAI ELKHAPGLIA ALAYETANFY QKADHTLSSL EPAYSAKWRK 240
YLHLKMCFYT AYAYCYHGET LLASDKCGEA IRSLQEAEKL YAKAEALCKE YGETKGPGPT 300
VKPSGHLFFR KLGNLVKNTL EKCQRENGFI YFQKIPTEAP QLELKANYGL VEPIPFEFPP 360
TSVQWTPETL AAFDLTKRPK DDSTKPKPEE EVKPVKEPDI KPQKDTGCYI S 411 
Gene Ontology
 GO:0016020; C:membrane; IEA:UniProtKB-SubCell. 
Interpro
 IPR004328; BRO1_dom. 
Pfam
 PF03097; BRO1 
SMART
 SM01041; BRO1 
PROSITE
 PS51180; BRO1 
PRINTS