CPLM-000547

Genbank Nucleotide ID

Protein Synonyms/Alias

B-cell receptor-associated protein BAP37; Repressor of estrogen receptor activity

Mus musculus (Mouse)

Position	Peptide	Type	References
6	**MAQNLKDLAGRLP	acetylation	[1]
6	**MAQNLKDLAGRLP	ubiquitination	[2]
97	ISSPTGSKDLQMVNI	acetylation	[3]
147	VLKSVVAKFNASQLI	acetylation	[3]
200	YTAAVEAKQVAQQEA	acetylation	[1, 3, 4, 5]
216	RAQFLVEKAKQEQRQ	acetylation	[3]
224	AKQEQRQKIVQAEGE	acetylation	[3]
224	AKQEQRQKIVQAEGE	ubiquitination	[2]
262	RAAQNISKTIATSQN	ubiquitination	[2]

[1] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
[2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
[3] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
[4] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
Simon GM, Cheng J, Gordon JI.
Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
[5] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
J Biol Chem. 2013 Jul 17;. [PMID: 23864654]

Functional Description

Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. Probably involved in regulating mitochondrial respiration activity and in aging.

REGION 19 49 Necessary for transcriptional repression
REGION 150 174 Necessary for transcriptional repression
MOD_RES 2 2 N-acetylalanine (By similarity).
MOD_RES 128 128 Phosphotyrosine (By similarity).
MOD_RES 250 250 N6-acetyllysine (By similarity).

Acetylation; Coiled coil; Complete proteome; Cytoplasm; Direct protein sequencing; Membrane; Mitochondrion; Mitochondrion inner membrane; Nucleus; Phosphoprotein; Receptor; Reference proteome; Repressor; Transcription; Transcription regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005743; C:mitochondrial inner membrane; IDA:UniProtKB.
GO:0005634; C:nucleus; IDA:UniProtKB.
GO:0060749; P:mammary gland alveolus development; IMP:MGI.
GO:0060744; P:mammary gland branching involved in thelarche; IMP:MGI.
GO:0033147; P:negative regulation of intracellular estrogen receptor signaling pathway; IMP:MGI.
GO:0033600; P:negative regulation of mammary gland epithelial cell proliferation; IMP:MGI.
GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:UniProtKB.
GO:0060762; P:regulation of branching involved in mammary gland duct morphogenesis; IMP:MGI.
GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.

IPR001107; Band_7.
IPR000163; Prohibitin.

PR00679; PROHIBITIN.