UniProt Accession

 SPED_ECOLI; P0A7F6; P09159 

Genbank Protein ID

 J02804; U00096; AP009048 

Genbank Nucleotide ID

 AAA24644.1; AAC73231.1; BAB96694.1 

Protein Name

 S-adenosylmethionine decarboxylase proenzyme 

Protein Synonyms/Alias

 AdoMetDC; SAMDC; S-adenosylmethionine decarboxylase beta chain; S-adenosylmethionine decarboxylase alpha chain 

Gene Name

 speD 

Gene Synonyms/Alias

 b0120; JW0116 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
5	***MKKLKLHGFNNL	acetylation	[1]
90	SEEPVDPKLIDKTEH	acetylation	[1]
94	VDPKLIDKTEHPGPL	acetylation	[1]
220	FHTKMLLKEFDLKHY	acetylation	[1]
225	LLKEFDLKHYMFHTK	acetylation	[1, 2]
232	KHYMFHTKPEDLTDS	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 

Functional Description

 Catalyzes the decarboxylation of S-adenosylmethionine to S-adenosylmethioninamine (dcAdoMet), the propylamine donor required for the synthesis of the polyamines spermine and spermidine from the diamine putrescine. 

Sequence Annotation

 ACT_SITE 112 112 Schiff-base intermediate with substrate;
 ACT_SITE 117 117 Proton acceptor; for processing activity
 ACT_SITE 140 140 Proton donor; for catalytic activity (By
 MOD_RES 112 112 Pyruvic acid (Ser); by autocatalysis.  

Keyword

 Allosteric enzyme; Autocatalytic cleavage; Complete proteome; Decarboxylase; Direct protein sequencing; Lyase; Magnesium; Polyamine biosynthesis; Pyruvate; Reference proteome; S-adenosyl-L-methionine; Schiff base; Spermidine biosynthesis; Zymogen. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 264 AA 

Protein Sequence

Gene Ontology

 GO:0004014; F:adenosylmethionine decarboxylase activity; IDA:EcoCyc.
 GO:0006557; P:S-adenosylmethioninamine biosynthetic process; IEA:HAMAP.
 GO:0008295; P:spermidine biosynthetic process; IMP:EcoCyc. 

Interpro

 IPR003826; AdoMetDC_fam_prok.
 IPR009165; S-AdoMet_deCO2ase_bac.
 IPR016067; S-AdoMet_deCO2ase_core. 

Pfam

 PF02675; AdoMet_dc 

SMART

  

PROSITE

  

PRINTS