CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002500
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phosphoenolpyruvate carboxykinase, cytosolic [GTP] 
Protein Synonyms/Alias
 PEPCK-C; Phosphoenolpyruvate carboxylase 
Gene Name
 Pck1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
243GGNSLLGKKCFALRIubiquitination[1]
342VAPGTSVKTNPNAIKubiquitination[1]
349KTNPNAIKTIQKNTIubiquitination[1]
521VNWFRKDKNGKFLWPacetylation[2]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. 
Sequence Annotation
 NP_BIND 287 292 GTP.
 NP_BIND 530 533 GTP.
 REGION 403 405 Substrate binding.
 ACT_SITE 288 288
 METAL 244 244 Manganese.
 METAL 264 264 Manganese.
 METAL 311 311 Manganese.
 BINDING 87 87 Substrate.
 BINDING 237 237 Substrate; via amide nitrogen.
 BINDING 244 244 Substrate.
 BINDING 286 286 Substrate.
 BINDING 405 405 GTP.
 BINDING 436 436 GTP.
 MOD_RES 70 70 N6-acetyllysine; by p300/EP300 (By
 MOD_RES 71 71 N6-acetyllysine; by p300/EP300 (By
 MOD_RES 594 594 N6-acetyllysine; by p300/EP300 (By  
Keyword
 3D-structure; Acetylation; Complete proteome; Cytoplasm; Decarboxylase; Direct protein sequencing; Gluconeogenesis; GTP-binding; Lyase; Manganese; Metal-binding; Nucleotide-binding; Reference proteome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 622 AA 
Protein Sequence
MPPQLHNGLD FSAKVIQGSL DSLPQEVRKF VEGNAQLCQP EYIHICDGSE EEYGRLLAHM 60
QEEGVIRKLK KYDNCWLALT DPRDVARIES KTVIITQEQR DTVPIPKSGQ SQLGRWMSEE 120
DFEKAFNARF PGCMKGRTMY VIPFSMGPLG SPLAKIGIEL TDSPYVVASM RIMTRMGTSV 180
LEALGDGEFI KCLHSVGCPL PLKKPLVNNW ACNPELTLIA HLPDRREIIS FGSGYGGNSL 240
LGKKCFALRI ASRLAKEEGW LAEHMLILGI TNPEGKKKYL AAAFPSACGK TNLAMMNPTL 300
PGWKVECVGD DIAWMKFDAQ GNLRAINPEN GFFGVAPGTS VKTNPNAIKT IQKNTIFTNV 360
AETSDGGVYW EGIDEPLAPG VTITSWKNKE WRPQDEEPCA HPNSRFCTPA SQCPIIDPAW 420
ESPEGVPIEG IIFGGRRPAG VPLVYEALSW QHGVFVGAAM RSEATAAAEH KGKVIMHDPF 480
AMRPFFGYNF GKYLAHWLSM AHRPAAKLPK IFHVNWFRKD KNGKFLWPGF GENSRVLEWM 540
FGRIEGEDSA KLTPIGYVPK EDALNLKGLG DVNVEELFGI SKEFWEKEVE EIDKYLEDQV 600
NADLPYEIER ELRALKQRIS QM 622 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0031406; F:carboxylic acid binding; IDA:BHF-UCL.
 GO:0019003; F:GDP binding; IDA:RGD.
 GO:0005525; F:GTP binding; IDA:BHF-UCL.
 GO:0000287; F:magnesium ion binding; ISS:BHF-UCL.
 GO:0030145; F:manganese ion binding; IDA:BHF-UCL.
 GO:0004613; F:phosphoenolpyruvate carboxykinase (GTP) activity; IDA:BHF-UCL.
 GO:0006094; P:gluconeogenesis; IDA:BHF-UCL.
 GO:0042593; P:glucose homeostasis; IDA:BHF-UCL.
 GO:0046327; P:glycerol biosynthetic process from pyruvate; IDA:BHF-UCL.
 GO:0006475; P:internal protein amino acid acetylation; ISS:UniProtKB.
 GO:0006107; P:oxaloacetate metabolic process; IDA:RGD.
 GO:0014823; P:response to activity; IEP:RGD.
 GO:0032868; P:response to insulin stimulus; ISS:BHF-UCL. 
Interpro
 IPR018091; PEP_carboxykin_GTP_CS.
 IPR013035; PEP_carboxykinase_C.
 IPR008209; PEP_carboxykinase_GTP.
 IPR008210; PEP_carboxykinase_N. 
Pfam
 PF00821; PEPCK 
SMART
  
PROSITE
 PS00505; PEPCK_GTP 
PRINTS