UniProt Accession

 END8_ECOLI; P50465 

Genbank Protein ID

 U38616; D89754; U00096; AP009048 

Genbank Nucleotide ID

 AAC45355.1; BAA20414.1; AAC73808.1; BAA35378.1 

Protein Name

 Endonuclease 8 

Protein Synonyms/Alias

 DNA glycosylase/AP lyase Nei; DNA-(apurinic or apyrimidinic site) lyase Nei; Endonuclease VIII 

Gene Name

 nei 

Gene Synonyms/Alias

 b0714; JW0704 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
220	RGQVDENKHHGALFR	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, 5,6-dihydrouracil and 5,6-dihydrothymine. Acts on DNA bubble and 3'-fork structures, suggesting a role in replication-associated DNA repair. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. 

Sequence Annotation

 ZN_FING 229 263 FPG-type.
 ACT_SITE 2 2 Schiff-base intermediate with DNA.
 ACT_SITE 3 3 Proton donor (Probable).
 ACT_SITE 53 53 Proton donor; for beta-elimination
 ACT_SITE 253 253 Proton donor; for delta-elimination
 BINDING 70 70 DNA.
 BINDING 125 125 DNA.
 BINDING 169 169 DNA.  

Keyword

 3D-structure; Complete proteome; Direct protein sequencing; DNA damage; DNA repair; DNA-binding; Glycosidase; Hydrolase; Lyase; Metal-binding; Multifunctional enzyme; Reference proteome; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 263 AA 

Protein Sequence

Gene Ontology

 GO:0003684; F:damaged DNA binding; IDA:EcoliWiki.
 GO:0003906; F:DNA-(apurinic or apyrimidinic site) lyase activity; IDA:EcoliWiki.
 GO:0004519; F:endonuclease activity; IEA:InterPro.
 GO:0000703; F:oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity; IEA:HAMAP.
 GO:0008270; F:zinc ion binding; IDA:EcoliWiki.
 GO:0006284; P:base-excision repair; IDA:EcoliWiki.
 GO:0006289; P:nucleotide-excision repair; IEA:InterPro. 

Interpro

 IPR015886; DNA_glyclase/AP_lyase_DNA-bd.
 IPR015887; DNA_glyclase_Znf_dom_DNA_BS.
 IPR012319; DNA_glycosylase/AP_lyase_cat.
 IPR023713; Endonuclease-VIII.
 IPR010979; Ribosomal_S13-like_H2TH.
 IPR000214; Znf_DNA_glyclase/AP_lyase.
 IPR010663; Znf_DNA_glyclase/IsotRNA_synth. 

Pfam

 PF01149; Fapy_DNA_glyco
 PF06831; H2TH
 PF06827; zf-FPG_IleRS 

SMART

 SM00898; Fapy_DNA_glyco 

PROSITE

 PS51068; FPG_CAT
 PS01242; ZF_FPG_1
 PS51066; ZF_FPG_2 

PRINTS