CPLM-009150

Genbank Nucleotide ID

Histone H2B type 1-D

Protein Synonyms/Alias

HIRA-interacting protein 2; Histone H2B.1 B; Histone H2B.b; H2B/b

Homo sapiens (Human)

Position	Peptide	Type	References
6	**MPEPTKSAPAPKK	acetylation	[1, 2, 3]
6	**MPEPTKSAPAPKK	ubiquitination	[3, 4, 5]
12	TKSAPAPKKGSKKAV	acetylation	[2, 3]
13	KSAPAPKKGSKKAVT	acetylation	[2]
16	PAPKKGSKKAVTKAQ	acetylation	[2]
35	KKRKRSRKESYSVYV	ubiquitination	[6]
44	SYSVYVYKVLKQVHP	ubiquitination	[6]
47	VYVYKVLKQVHPDTG	methylation	[7]
47	VYVYKVLKQVHPDTG	ubiquitination	[6]
58	PDTGISSKAMGIMNS	methylation	[7]
58	PDTGISSKAMGIMNS	ubiquitination	[6]
109	LLPGELAKHAVSEGT	methylation	[7]
109	LLPGELAKHAVSEGT	ubiquitination	[6]
117	HAVSEGTKAVTKYTS	ubiquitination	[6]
121	EGTKAVTKYTSSK**	ubiquitination	[6]

[1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[2] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224]
[3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[7] Update on activities at the Universal Protein Resource (UniProt) in 2013.
e="String">UniProt Consortium.
Nucleic Acids Res. 2013 Jan;41(Database issue):D43-7. [PMID: 23161681]

Functional Description

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

MOD_RES 2 2 N-acetylproline (By similarity).
MOD_RES 6 6 N6-acetyllysine; alternate.
MOD_RES 6 6 N6-crotonyl-L-lysine; alternate.
MOD_RES 12 12 N6-acetyllysine; alternate.
MOD_RES 12 12 N6-crotonyl-L-lysine; alternate.
MOD_RES 13 13 N6-acetyllysine; alternate.
MOD_RES 13 13 N6-crotonyl-L-lysine; alternate.
MOD_RES 15 15 Phosphoserine; by STK4/MST1.
MOD_RES 16 16 N6-acetyllysine; alternate.
MOD_RES 16 16 N6-crotonyl-L-lysine; alternate.
MOD_RES 17 17 N6-acetyllysine; alternate.
MOD_RES 17 17 N6-crotonyl-L-lysine; alternate.
MOD_RES 21 21 N6-acetyllysine; alternate.
MOD_RES 21 21 N6-crotonyl-L-lysine; alternate.
MOD_RES 24 24 N6-acetyllysine; alternate (By
MOD_RES 24 24 N6-crotonyl-L-lysine; alternate.
MOD_RES 35 35 N6-crotonyl-L-lysine; alternate.
MOD_RES 37 37 Phosphoserine; by AMPK (By similarity).
MOD_RES 43 43 Phosphotyrosine (By similarity).
MOD_RES 47 47 N6-methyllysine.
MOD_RES 58 58 N6,N6-dimethyllysine.
MOD_RES 109 109 N6-methyllysine.
MOD_RES 113 113 Phosphoserine; alternate (By similarity).
CARBOHYD 113 113 O-linked (GlcNAc...); alternate (By
CROSSLNK 35 35 Glycyl lysine isopeptide (Lys-Gly)
CROSSLNK 121 121 Glycyl lysine isopeptide (Lys-Gly)

Acetylation; Chromosome; Complete proteome; Direct protein sequencing; DNA-binding; Glycoprotein; Isopeptide bond; Methylation; Nucleosome core; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0000786; C:nucleosome; IEA:UniProtKB-KW.
GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
GO:0006334; P:nucleosome assembly; IEA:InterPro.

IPR009072; Histone-fold.
IPR007125; Histone_core_D.
IPR000558; Histone_H2B.

PS00357; HISTONE_H2B

PR00621; HISTONEH2B.