CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011618
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Multisubstrate pseudouridine synthase 7 
Protein Synonyms/Alias
 RNA pseudouridylate synthase 7; RNA-uridine isomerase 7; tRNA pseudouridine(13) synthase 
Gene Name
 PUS7 
Gene Synonyms/Alias
 YOR243C; O5254 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
10DSSEATVKRPLDAHVacetylation[1]
25GPSENAAKKLKIEQRacetylation[1]
229FTLHKENKDTMEAVNacetylation[1]
269CQRVSISKIGLDRLNacetylation[1]
582SYRTVIQKPKSLEYRubiquitination[2]
614ILNNTRAKESGQKYMubiquitination[3]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 
Functional Description
 Catalyzes pseudouridylation at position 35 in U2 snRNA stem-loop II region which induces particular conformation of the mRNA-U2 snRNA duplex and places the nucleophile in an accessible position for the first step of splicing. Catalyzes also pseudouridylation at position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr). Pseudouridine residues in tRNAs may stabilize the local RNA conformation, favor interactions with protein partners and play an important role in the stabilization of the codon-anticodon interaction with mRNA. 
Sequence Annotation
 DOMAIN 338 582 TRUD.
 ACT_SITE 256 256 Nucleophile.  
Keyword
 Complete proteome; Isomerase; mRNA processing; mRNA splicing; Nucleus; Reference proteome; Ribosome biogenesis; rRNA processing; tRNA processing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 676 AA 
Protein Sequence
MSDSSEATVK RPLDAHVGPS ENAAKKLKIE QRTQADGIHE ADVGITLFLS PELPGFRGQI 60
KQRYTDFLVN EIDQEGKVIH LTDKGFKMPK KPQRSKEEVN AEKESEAARR QEFNVDPELR 120
NQLVEIFGEE DVLKIESVYR TANKMETAKN FEDKSVRTKI HQLLREAFKN ELESVTTDTN 180
TFKIARSNRN SRTNKQEKIN QTRDANGVEN WGYGPSKDFI HFTLHKENKD TMEAVNVITK 240
LLRVPSRVIR YAGTKDRRAV TCQRVSISKI GLDRLNALNR TLKGMIIGNY NFSDASLNLG 300
DLKGNEFVVV IRDVTTGNSE VSLEEIVSNG CKSLSENGFI NYFGMQRFGT FSISTHTIGR 360
ELLLSNWKKA AELILSDQDN VLPKSKEARK IWAETKDAAL ALKQMPRQCL AENALLYSLS 420
NQRKEEDGTY SENAYYTAIM KIPRNLRTMY VHAYQSYVWN SIASKRIELH GLKLVVGDLV 480
IDTSEKSPLI SGIDDEDFDE DVREAQFIRA KAVTQEDIDS VKYTMEDVVL PSPGFDVLYP 540
SNEELKQLYV DILKADNMDP FNMRRKVRDF SLAGSYRTVI QKPKSLEYRI IHYDDPSQQL 600
VNTDLDILNN TRAKESGQKY MKAKLDRYMP DKGGEKTAVV LKFQLGTSAY ATMALRELMK 660
LETSRRGDMC DVKENI 676 
Gene Ontology
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0009982; F:pseudouridine synthase activity; IDA:SGD.
 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IMP:SGD.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
 GO:0031120; P:snRNA pseudouridine synthesis; IDA:SGD.
 GO:0031119; P:tRNA pseudouridine synthesis; IMP:SGD. 
Interpro
 IPR020103; PsdUridine_synth_cat_dom.
 IPR001656; PsdUridine_synth_TruD.
 IPR020119; PsdUridine_synth_TruD_CS.
 IPR017091; PsdUridine_synth_TruD_euk.
 IPR011760; PsdUridine_synth_TruD_insert. 
Pfam
 PF01142; TruD 
SMART
  
PROSITE
 PS50984; TRUD
 PS01268; UPF0024 
PRINTS