CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001823
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Citrate synthase, mitochondrial 
Protein Synonyms/Alias
  
Gene Name
 CIT1 
Gene Synonyms/Alias
 GLU3; LYS6; YNR001C; N2019 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
88YGGMRGIKGLVWEGSacetylation[1]
195ESESKFAKAYAQGVSacetylation[1]
227KLPVIASKIYRNVFKacetylation[1]
237RNVFKDGKITSTDPNubiquitination[2]
249DPNADYGKNLAQLLGacetylation[1]
331FKLREEVKGDYSKETacetylation[1]
336EVKGDYSKETIEKYLacetylation[1]
377AQREFALKHFPDYELacetylation[1]
401VAPGVLTKHGKTKNPacetylation[1]
461GAPIERPKSFSTEKYacetylation[1]
467PKSFSTEKYKELVKKacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
  
Sequence Annotation
 ACT_SITE 312 312 By similarity.
 ACT_SITE 358 358 By similarity.
 ACT_SITE 413 413 By similarity.  
Keyword
 Complete proteome; Direct protein sequencing; Mitochondrion; Reference proteome; Transferase; Transit peptide; Tricarboxylic acid cycle. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 479 AA 
Protein Sequence
MSAILSTTSK SFLSRGSTRQ CQNMQKALFA LLNARHYSSA SEQTLKERFA EIIPAKAEEI 60
KKFKKEHGKT VIGEVLLEQA YGGMRGIKGL VWEGSVLDPE EGIRFRGRTI PEIQRELPKA 120
EGSTEPLPEA LFWLLLTGEI PTDAQVKALS ADLAARSEIP EHVIQLLDSL PKDLHPMAQF 180
SIAVTALESE SKFAKAYAQG VSKKEYWSYT FEDSLDLLGK LPVIASKIYR NVFKDGKITS 240
TDPNADYGKN LAQLLGYENK DFIDLMRLYL TIHSDHEGGN VSAHTTHLVG SALSSPYLSL 300
AAGLNGLAGP LHGRANQEVL EWLFKLREEV KGDYSKETIE KYLWDTLNAG RVVPGYGHAV 360
LRKTDPRYTA QREFALKHFP DYELFKLVST IYEVAPGVLT KHGKTKNPWP NVDSHSGVLL 420
QYYGLTEASF YTVLFGVARA IGVLPQLIID RAVGAPIERP KSFSTEKYKE LVKKIESKN 479 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005758; C:mitochondrial intermembrane space; TAS:Reactome.
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0004108; F:citrate (Si)-synthase activity; IMP:SGD.
 GO:0046356; P:acetyl-CoA catabolic process; IMP:SGD.
 GO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro.
 GO:0006101; P:citrate metabolic process; TAS:SGD.
 GO:0006537; P:glutamate biosynthetic process; TAS:SGD.
 GO:0006099; P:tricarboxylic acid cycle; TAS:SGD. 
Interpro
 IPR016142; Citrate_synth-like_lrg_a-sub.
 IPR002020; Citrate_synthase-like.
 IPR016141; Citrate_synthase-like_core.
 IPR019810; Citrate_synthase_AS.
 IPR010109; Citrate_synthase_euk. 
Pfam
 PF00285; Citrate_synt 
SMART
  
PROSITE
 PS00480; CITRATE_SYNTHASE 
PRINTS
 PR00143; CITRTSNTHASE.