UniProt Accession

 NNMT_HUMAN; P40261 

Genbank Protein ID

 U08021; U20971; U20970; BC000234 

Genbank Nucleotide ID

 AAA19904.1; AAA93158.1; AAA93158.1; AAH00234.1 

Protein Name

 Nicotinamide N-methyltransferase 

Protein Synonyms/Alias

  

Gene Name

 NNMT 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
8	MESGFTSKDTYLSHF	methylation	[1]
8	MESGFTSKDTYLSHF	ubiquitination	[2]
23	NPRDYLEKYYKFGSR	ubiquitination	[2]
39	SAESQILKHLLKNLF	acetylation	[3, 4]
43	QILKHLLKNLFKIFC	acetylation	[4]
43	QILKHLLKNLFKIFC	ubiquitination	[2]
47	HLLKNLFKIFCLDGV	ubiquitination	[2]
210	YYMIGEQKFSSLPLG	ubiquitination	[2]

Reference

 [1] Large-scale global identification of protein lysine methylation in vivo.
 Cao XJ, Arnaudo AM, Garcia BA.
 Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773] 

Functional Description

 Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. 

Sequence Annotation

 REGION 63 64 S-adenosyl-L-methionine binding.
 REGION 142 143 S-adenosyl-L-methionine binding.
 BINDING 20 20 S-adenosyl-L-methionine.
 BINDING 25 25 S-adenosyl-L-methionine.
 BINDING 69 69 S-adenosyl-L-methionine.
 BINDING 85 85 S-adenosyl-L-methionine.
 BINDING 90 90 S-adenosyl-L-methionine.
 BINDING 163 163 S-adenosyl-L-methionine.
 MOD_RES 39 39 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Methyltransferase; Reference proteome; S-adenosyl-L-methionine; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 264 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0008112; F:nicotinamide N-methyltransferase activity; TAS:ProtInc.
 GO:0031100; P:organ regeneration; IEA:Compara.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0010243; P:response to organic nitrogen; IEA:Compara.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0006805; P:xenobiotic metabolic process; TAS:Reactome. 

Interpro

 IPR025818; NNMT.
 IPR025820; NNMT/PNMT/TEMT_CS.
 IPR000940; NNMT_TEMT_trans. 

Pfam

 PF01234; NNMT_PNMT_TEMT 

SMART

  

PROSITE

 PS01100; NNMT_PNMT_TEMT 

PRINTS