CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010457
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 T-complex protein 1 subunit epsilon 
Protein Synonyms/Alias
 TCP-1-epsilon; CCT-epsilon 
Gene Name
 Cct5 
Gene Synonyms/Alias
 Ccte; Kiaa0098 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
20GRPFLIIKDQDRKSRubiquitination[1]
35LMGLEALKSHIMAAKacetylation[2]
59LGPNGLDKMMVDKDGubiquitination[1]
64LDKMMVDKDGDVTITubiquitination[1]
150IAIQHLDKISDKVLVubiquitination[1]
154HLDKISDKVLVDINNubiquitination[1]
170EPLIQTAKTTLGSKVubiquitination[1]
210DVDFELIKVEGKVGGubiquitination[1]
223GGRLEDTKLIKGVIVacetylation[3]
232IKGVIVDKDFSHPQMacetylation[4]
259TCPFEPPKPKTKHKLacetylation[2]
265PKPKTKHKLDVMSVEacetylation[4]
265PKPKTKHKLDVMSVEubiquitination[1]
275VMSVEDYKALQKYEKubiquitination[1]
370SFGTTKDKMLVIEKCacetylation[3]
392IFIRGGNKMIIEEAKubiquitination[1]
529QMVRMILKIDDIRKPacetylation[2]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
 Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin (By similarity). 
Sequence Annotation
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 26 26 Phosphoserine (By similarity).  
Keyword
 Acetylation; ATP-binding; Chaperone; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Nucleotide-binding; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 541 AA 
Protein Sequence
MASVGTLAFD EYGRPFLIIK DQDRKSRLMG LEALKSHIMA AKAVANTMRT SLGPNGLDKM 60
MVDKDGDVTI TNDGATILSM MDVDHQIAKL MVELSKSQDD EIGDGTTGVV VLAGALLEEA 120
EQLLDRGIHP IRIADGYEQA ARIAIQHLDK ISDKVLVDIN NPEPLIQTAK TTLGSKVINS 180
CHRQMAEIAV NAVLTVADME RRDVDFELIK VEGKVGGRLE DTKLIKGVIV DKDFSHPQMP 240
KKVVDAKIAI LTCPFEPPKP KTKHKLDVMS VEDYKALQKY EKEKFEEMIK QIKETGANLA 300
ICQWGFDDEA NHLLLQNGLP AVRWVGGPEI ELIAIATGGR IVPRFSELTS EKLGFAGVVQ 360
EISFGTTKDK MLVIEKCKNS RAVTIFIRGG NKMIIEEAKR SLHDALCVIR NLIRDNRVVY 420
GGGAAEISCA LAVSQEADKC PTLEQYAMRA FADALEVIPM ALSENSGMNP IQTMTEVRAR 480
QVKESNPALG IDCLHKGSND MQYQHVIETL IGKKQQISLA TQMVRMILKI DDIRKPGESE 540
E 541 
Gene Ontology
 GO:0044297; C:cell body; IDA:MGI.
 GO:0005813; C:centrosome; IEA:Compara.
 GO:0005832; C:chaperonin-containing T-complex; IDA:MGI.
 GO:0005874; C:microtubule; IEA:Compara.
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0002199; C:zona pellucida receptor complex; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0007339; P:binding of sperm to zona pellucida; IDA:MGI.
 GO:0006457; P:protein folding; IEA:InterPro.
 GO:0009615; P:response to virus; IEA:Compara. 
Interpro
 IPR012718; Chap_CCT_epsi.
 IPR017998; Chaperone_TCP-1.
 IPR002194; Chaperonin_TCP-1_CS.
 IPR002423; Cpn60/TCP-1.
 IPR027409; GroEL-like_apical_dom.
 IPR027413; GROEL-like_equatorial.
 IPR027410; TCP-1-like_intermed. 
Pfam
 PF00118; Cpn60_TCP1 
SMART
  
PROSITE
 PS00750; TCP1_1
 PS00751; TCP1_2
 PS00995; TCP1_3 
PRINTS
 PR00304; TCOMPLEXTCP1.