CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004770
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ETS domain-containing protein Elk-1 
Protein Synonyms/Alias
  
Gene Name
 ELK1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
230PPEAPNLKSEELNVEsumoylation[1, 2, 3, 4, 5]
249RALPPEVKVEGPKEEsumoylation[1, 2, 3, 4, 5]
254EVKVEGPKEELEVAGsumoylation[3]
Reference
 [1] Dynamic interplay of the SUMO and ERK pathways in regulating Elk-1 transcriptional activity.
 Yang SH, Jaffray E, Hay RT, Sharrocks AD.
 Mol Cell. 2003 Jul;12(1):63-74. [PMID: 12887893]
 [2] SUMO promotes HDAC-mediated transcriptional repression.
 Yang SH, Sharrocks AD.
 Mol Cell. 2004 Feb 27;13(4):611-7. [PMID: 14992729]
 [3] SUMOylation regulates nucleo-cytoplasmic shuttling of Elk-1.
 Salinas S, Briançon-Marjollet A, Bossis G, Lopez MA, Piechaczyk M, Jariel-Encontre I, Debant A, Hipskind RA.
 J Cell Biol. 2004 Jun 21;165(6):767-73. [PMID: 15210726]
 [4] Convergence of the SUMO and MAPK pathways on the ETS-domain transcription factor Elk-1.
 Yang SH, Sharrocks AD.
 Biochem Soc Symp. 2006;(73):121-9. [PMID: 16626293]
 [5] Growth factor-induced phosphorylation of sterol regulatory element-binding proteins inhibits sumoylation, thereby stimulating the expression of their target genes, low density lipoprotein uptake, and lipid synthesis.
 Arito M, Horiba T, Hachimura S, Inoue J, Sato R.
 J Biol Chem. 2008 May 30;283(22):15224-31. [PMID: 18403372] 
Functional Description
 Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element. 
Sequence Annotation
 DNA_BIND 5 86 ETS.
 REGION 349 399 Sufficient for interaction with MAD2L2.
 MOD_RES 324 324 Phosphoserine; by MAPK1.
 MOD_RES 336 336 Phosphothreonine; by MAPK1.
 MOD_RES 353 353 Phosphothreonine; by MAPK1.
 MOD_RES 363 363 Phosphothreonine; by MAPK1.
 MOD_RES 368 368 Phosphothreonine; by MAPK1.
 MOD_RES 383 383 Phosphoserine; by MAPK1 and MAPK8.
 MOD_RES 389 389 Phosphoserine; by MAPK1.
 MOD_RES 417 417 Phosphothreonine; by MAPK1.
 MOD_RES 422 422 Phosphoserine; by MAPK1.
 CROSSLNK 230 230 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 249 249 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 254 254 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Activator; Alternative splicing; Complete proteome; Direct protein sequencing; DNA-binding; Isopeptide bond; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 428 AA 
Protein Sequence
MDPSVTLWQF LLQLLREQGN GHIISWTSRD GGEFKLVDAE EVARLWGLRK NKTNMNYDKL 60
SRALRYYYDK NIIRKVSGQK FVYKFVSYPE VAGCSTEDCP PQPEVSVTST MPNVAPAAIH 120
AAPGDTVSGK PGTPKGAGMA GPGGLARSSR NEYMRSGLYS TFTIQSLQPQ PPPHPRPAVV 180
LPSAAPAGAA APPSGSRSTS PSPLEACLEA EEAGLPLQVI LTPPEAPNLK SEELNVEPGL 240
GRALPPEVKV EGPKEELEVA GERGFVPETT KAEPEVPPQE GVPARLPAVV MDTAGQAGGH 300
AASSPEISQP QKGRKPRDLE LPLSPSLLGG PGPERTPGSG SGSGLQAPGP ALTPSLLPTH 360
TLTPVLLTPS SLPPSIHFWS TLSPIAPRSP AKLSFQFPSS GSAQVHIPSI SVDGLSTPVV 420
LSPGPQKP 428 
Gene Ontology
 GO:0005634; C:nucleus; IC:UniProtKB.
 GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:UniProtKB.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0002755; P:MyD88-dependent toll-like receptor signaling pathway; TAS:Reactome.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0051403; P:stress-activated MAPK cascade; TAS:Reactome.
 GO:0034166; P:toll-like receptor 10 signaling pathway; TAS:Reactome.
 GO:0034134; P:toll-like receptor 2 signaling pathway; TAS:Reactome.
 GO:0034138; P:toll-like receptor 3 signaling pathway; TAS:Reactome.
 GO:0034142; P:toll-like receptor 4 signaling pathway; TAS:Reactome.
 GO:0034146; P:toll-like receptor 5 signaling pathway; TAS:Reactome.
 GO:0034162; P:toll-like receptor 9 signaling pathway; TAS:Reactome.
 GO:0038123; P:toll-like receptor TLR1:TLR2 signaling pathway; TAS:Reactome.
 GO:0038124; P:toll-like receptor TLR6:TLR2 signaling pathway; TAS:Reactome.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0035666; P:TRIF-dependent toll-like receptor signaling pathway; TAS:Reactome. 
Interpro
 IPR000418; Ets_dom.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF00178; Ets 
SMART
 SM00413; ETS 
PROSITE
 PS00345; ETS_DOMAIN_1
 PS00346; ETS_DOMAIN_2
 PS50061; ETS_DOMAIN_3 
PRINTS
 PR00454; ETSDOMAIN.