UniProt Accession

 NR2C1_HUMAN; P13056; A8K5K4; Q15625; Q15626 

Genbank Protein ID

 M21985; M29959; M29960; AK291319; AC011598; CH471054; BC040141 

Genbank Nucleotide ID

 AAA36650.1; AAA36762.1; AAA36761.1; BAF84008.1; EAW97518.1; AAH40141.1 

Protein Name

 Nuclear receptor subfamily 2 group C member 1 

Protein Synonyms/Alias

 Orphan nuclear receptor TR2; Testicular receptor 2 

Gene Name

 NR2C1 

Gene Synonyms/Alias

 TR2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
119	LCVVCGDKASGRHYG	ubiquitination	[1]
538	EFQDYITKTYPDDTY	ubiquitination	[2, 3]

Reference

 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Orphan nuclear receptor. Binds the IR7 element in the promoter of its own gene in an autoregulatory negative feedback mechanism. Primarily repressor of a broad range of genes. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Together with NR2C2, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription. Also activator of OCT4 gene expression. May be involved in stem cell proliferation and differentiation. Mediator of retinoic acid- regulated preadipocyte proliferation. 

Sequence Annotation

 DNA_BIND 110 185 Nuclear receptor.
 ZN_FING 113 133 NR C4-type.
 ZN_FING 149 173 NR C4-type.
 REGION 1 178 Required for interaction with KAT2B (By
 REGION 584 603 Required for interaction with NRIP1 (By
 MOD_RES 197 197 Phosphoserine (By similarity).
 MOD_RES 215 215 Phosphoserine.
 MOD_RES 220 220 Phosphothreonine.
 MOD_RES 222 222 Phosphothreonine; by MAPK1 (By
 MOD_RES 581 581 Phosphoserine; by PKC (By similarity).
 CROSSLNK 250 250 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Activator; Alternative splicing; Complete proteome; DNA-binding; Isopeptide bond; Metal-binding; Nucleus; Phosphoprotein; Receptor; Reference proteome; Repressor; Transcription; Transcription regulation; Ubl conjugation; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 603 AA 

Protein Sequence

Gene Ontology

 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0016605; C:PML body; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; TAS:ProtInc.
 GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro.
 GO:0003707; F:steroid hormone receptor activity; TAS:ProtInc.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0048386; P:positive regulation of retinoic acid receptor signaling pathway; IEA:Compara.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome. 

Interpro

 IPR008946; Nucl_hormone_rcpt_ligand-bd.
 IPR000536; Nucl_hrmn_rcpt_lig-bd_core.
 IPR001723; Str_hrmn_rcpt.
 IPR001628; Znf_hrmn_rcpt.
 IPR013088; Znf_NHR/GATA. 

Pfam

 PF00104; Hormone_recep
 PF00105; zf-C4 

SMART

 SM00430; HOLI
 SM00399; ZnF_C4 

PROSITE

 PS00031; NUCLEAR_REC_DBD_1
 PS51030; NUCLEAR_REC_DBD_2 

PRINTS

 PR00398; STRDHORMONER.
 PR00047; STROIDFINGER.