CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029111
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 TNF receptor-associated factor 3 
Protein Synonyms/Alias
  
Gene Name
 TRAF3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
99QESIVKDKVFKDNCCubiquitination[1]
326WEEADSMKSSVESLQubiquitination[1, 2, 3, 4]
344TELESVDKSAGQVARubiquitination[1, 2, 3, 4, 5, 6]
488RHLGDAFKPDPNSSSubiquitination[2, 4, 6]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Metal-binding; Reference proteome; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 543 AA 
Protein Sequence
MESSKKMDSP GALQTNPPLK LHTDRSAGTP VFVPEQGGYK EKFVKTVEDK YKCEKCHLVL 60
CSPKQTECGH RFCESCMAAL LSSSSPKCTA CQESIVKDKV FKDNCCKREI LALQIYCRNE 120
SRGCAEQLML GHLLVHLKND CHFEELPCVR PDCKEKVLRK DLRDHVEKAC KYREATCSHC 180
KSQVPMIALQ KHEDTDCPCV VVSCPHKCSV QTLLRSEGTN QQIKAHEASS AVQHVNLLKE 240
WSNSLEKKVS LLQNESVEKN KSIQSLHNQI CSFEIEIERQ KEMLRNNESK ILHLQRVIDS 300
QAEKLKELDK EIRPFRQNWE EADSMKSSVE SLQNRVTELE SVDKSAGQVA RNTGLLESQL 360
SRHDQMLSVH DIRLADMDLR FQVLETASYN GVLIWKIRDY KRRKQEAVMG KTLSLYSQPF 420
YTGYFGYKMC ARVYLNGDGM GKGTHLSLFF VIMRGEYDAL LPWPFKQKVT LMLMDQGSSR 480
RHLGDAFKPD PNSSSFKKPT GEMNIASGCP VFVAQTVLEN GTYIKDDTIF IKVIVDTSDL 540
PDP 543 
Gene Ontology
 GO:0004842; F:ubiquitin-protein ligase activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0045087; P:innate immune response; IEA:InterPro.
 GO:0032088; P:negative regulation of NF-kappaB transcription factor activity; IEA:InterPro.
 GO:0042981; P:regulation of apoptotic process; IEA:InterPro.
 GO:0001817; P:regulation of cytokine production; IEA:InterPro.
 GO:0050688; P:regulation of defense response to virus; IEA:InterPro.
 GO:0008063; P:Toll signaling pathway; IEA:InterPro.
 GO:0033209; P:tumor necrosis factor-mediated signaling pathway; IEA:InterPro. 
Interpro
 IPR002083; MATH.
 IPR013323; SIAH-type.
 IPR012227; TNF_rcpt--assoc_TRAF.
 IPR008974; TRAF-like.
 IPR027128; TRAF3.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD.
 IPR017907; Znf_RING_CS.
 IPR001293; Znf_TRAF. 
Pfam
 PF00917; MATH 
SMART
 SM00061; MATH
 SM00184; RING 
PROSITE
 PS50144; MATH
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2
 PS50145; ZF_TRAF 
PRINTS