UniProt Accession

 SEC4_YEAST; P07560; D6VTM4 

Genbank Protein ID

 M16507; D50617; AY692843; BK006940 

Genbank Nucleotide ID

 AAA35032.1; BAA09233.1; AAT92862.1; DAA12434.1 

Protein Name

 Ras-related protein SEC4 

Protein Synonyms/Alias

 Suppressor of RHO3 protein 6 

Gene Name

 SEC4 

Gene Synonyms/Alias

 SRO6; YFL005W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
70	DINGKKVKLQLWDTA	acetylation	[1]
152	DQGEALAKELGIPFI	ubiquitination	[2]
183	LAKLIQEKIDSNKLV	acetylation	[1]
183	LAKLIQEKIDSNKLV	ubiquitination	[2]
196	LVGVGNGKEGNISIN	ubiquitination	[2]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Involved in exocytosis. Maybe by regulating the binding and fusion of secretory vesicles with the cell surface. The GTP- bound form of SEC4 may interact with an effector, thereby stimulating its activity and leading to exocytotic fusion. SEC4 may be an upstream activator of the 19.5S SEC8/SEC15 particle. SEC4 probably interacts directly with SEC8; it could serve as the attachment site for the SEC8/SEC15 particle. 

Sequence Annotation

 NP_BIND 27 34 GTP.
 NP_BIND 75 79 GTP.
 NP_BIND 133 136 GTP (By similarity).
 MOTIF 49 57 Effector region (Probable).
 MOD_RES 201 201 Phosphoserine.
 MOD_RES 204 204 Phosphoserine.
 LIPID 214 214 S-geranylgeranyl cysteine (By
 LIPID 215 215 S-geranylgeranyl cysteine (By  

Keyword

 3D-structure; Cell membrane; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Exocytosis; GTP-binding; Lipoprotein; Membrane; Nucleotide-binding; Phosphoprotein; Prenylation; Protein transport; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 215 AA 

Protein Sequence

Gene Ontology

 GO:0030478; C:actin cap; TAS:SGD.
 GO:0000131; C:incipient cellular bud site; IDA:SGD.
 GO:0005741; C:mitochondrial outer membrane; IDA:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0030133; C:transport vesicle; TAS:SGD.
 GO:0030658; C:transport vesicle membrane; IEA:UniProtKB-SubCell.
 GO:0005525; F:GTP binding; IDA:SGD.
 GO:0003924; F:GTPase activity; IDA:SGD.
 GO:0031321; P:ascospore-type prospore assembly; IMP:SGD.
 GO:0006914; P:autophagy; IMP:SGD.
 GO:0007121; P:bipolar cellular bud site selection; TAS:SGD.
 GO:0006887; P:exocytosis; IDA:SGD.
 GO:0006893; P:Golgi to plasma membrane transport; IMP:SGD.
 GO:0007107; P:membrane addition at site of cytokinesis; IMP:SGD.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0007264; P:small GTPase mediated signal transduction; TAS:SGD.
 GO:0006906; P:vesicle fusion; IMP:SGD. 

Interpro

 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR001806; Small_GTPase.
 IPR003579; Small_GTPase_Rab_type. 

Pfam

 PF00071; Ras 

SMART

 SM00175; RAB 

PROSITE

 PS51419; RAB 

PRINTS

 PR00449; RASTRNSFRMNG.