UniProt Accession

 SHPS1_RAT; P97710; O08951; O70426; Q9QWI5 

Genbank Protein ID

 D85183; D38468; U62328; AF055065 

Genbank Nucleotide ID

 BAA12734.1; BAA20368.1; AAC68478.1; AAC18089.1 

Protein Name

 Tyrosine-protein phosphatase non-receptor type substrate 1 

Protein Synonyms/Alias

 SHP substrate 1; SHPS-1; Brain Ig-like molecule with tyrosine-based activation motifs; Bit; CD172 antigen-like family member A; Inhibitory receptor SHPS-1; Macrophage fusion receptor; Macrophage membrane protein MFP150; Signal-regulatory protein alpha-1; Sirp-alpha-1; CD172a 

Gene Name

 Sirpa 

Gene Synonyms/Alias

 Bit; Mfr; Ptpns1; Shps1; Sirp 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
191	LKWLKDGKELSHLET	acetylation	[1]
417	TRLHEPEKNAREITQ	ubiquitination	[2]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 

Functional Description

 Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. May play a role in the release of nitric oxide by macrophages (By similarity). 

Sequence Annotation

 DOMAIN 32 138 Ig-like V-type.
 DOMAIN 150 248 Ig-like C1-type 1.
 DOMAIN 255 349 Ig-like C1-type 2.
 MOTIF 436 439 SH2-binding (Potential).
 MOTIF 446 451 SH3-binding (Potential).
 MOTIF 460 463 SH2-binding (Potential).
 MOTIF 477 480 SH2-binding (Potential).
 MOTIF 501 504 SH2-binding (Potential).
 MOD_RES 145 145 Phosphotyrosine (By similarity).
 MOD_RES 436 436 Phosphotyrosine; by Tyr-kinases
 MOD_RES 460 460 Phosphotyrosine; by Tyr-kinases
 MOD_RES 477 477 Phosphotyrosine; by Tyr-kinases.
 MOD_RES 501 501 Phosphotyrosine; by Tyr-kinases.
 CARBOHYD 54 54 N-linked (GlcNAc...) (Potential).
 CARBOHYD 93 93 N-linked (GlcNAc...) (Potential).
 CARBOHYD 169 169 N-linked (GlcNAc...) (Potential).
 CARBOHYD 181 181 N-linked (GlcNAc...) (Potential).
 CARBOHYD 205 205 N-linked (GlcNAc...) (Potential).
 CARBOHYD 209 209 N-linked (GlcNAc...) (Potential).
 CARBOHYD 242 242 N-linked (GlcNAc...) (Potential).
 CARBOHYD 246 246 N-linked (GlcNAc...) (Potential).
 CARBOHYD 271 271 N-linked (GlcNAc...) (Potential).
 CARBOHYD 293 293 N-linked (GlcNAc...) (Potential).
 CARBOHYD 312 312 N-linked (GlcNAc...) (Potential).
 CARBOHYD 320 320 N-linked (GlcNAc...) (Potential).
 CARBOHYD 345 345 N-linked (GlcNAc...) (Potential).
 DISULFID 55 122 Potential.
 DISULFID 172 229 Potential.
 DISULFID 274 332 Potential.  

Keyword

 Complete proteome; Direct protein sequencing; Disulfide bond; Glycoprotein; Immunoglobulin domain; Membrane; Phosphoprotein; Reference proteome; Repeat; SH3-binding; Signal; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 509 AA 

Protein Sequence

Gene Ontology

 GO:0005887; C:integral to plasma membrane; IEA:Compara.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0007015; P:actin filament organization; IEA:Compara.
 GO:0016477; P:cell migration; IDA:RGD.
 GO:0007160; P:cell-matrix adhesion; IEA:Compara.
 GO:0006911; P:phagocytosis, engulfment; IEA:Compara.
 GO:0006910; P:phagocytosis, recognition; IEA:Compara.
 GO:0050766; P:positive regulation of phagocytosis; IEA:Compara. 

Interpro

 IPR007110; Ig-like_dom.
 IPR013783; Ig-like_fold.
 IPR003006; Ig/MHC_CS.
 IPR003597; Ig_C1-set.
 IPR003599; Ig_sub.
 IPR013106; Ig_V-set. 

Pfam

 PF07654; C1-set
 PF07686; V-set 

SMART

 SM00409; IG
 SM00407; IGc1 

PROSITE

 PS50835; IG_LIKE
 PS00290; IG_MHC 

PRINTS