CPLM-019099

Genbank Nucleotide ID

Store-operated calcium entry-associated regulatory factor

Protein Synonyms/Alias

SARAF; SOCE-associated regulatory factor; HBV X-transactivated gene 3 protein; HBV XAg-transactivated protein 3; Protein FOAP-7; Transmembrane protein 66

SARAF; XTP3; HSPC035; NPD003; PSEC0019; UNQ1967/PRO4499

Homo sapiens (Human)

Position	Peptide	Type	References
43	RMLLRDVKALTLHYD	ubiquitination	[1, 2, 3, 4]
65	LDPIPQLKCVGGTAG	ubiquitination	[4]
79	GCDSYTPKVIQCQNK	ubiquitination	[2, 4]
86	KVIQCQNKGWDGYDV	ubiquitination	[4]
106	TDLDIAYKFGKTVVS	ubiquitination	[1, 2, 4, 5]
109	DIAYKFGKTVVSCEG	ubiquitination	[2, 4, 6]
145	YTELGLQKLKESGKQ	ubiquitination	[2, 4]
147	ELGLQKLKESGKQHG	ubiquitination	[4]
151	QKLKESGKQHGFASF	ubiquitination	[2]
326	VCSNSDTKTRTASGY	ubiquitination	[2, 3, 4, 5, 6]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]

Functional Description

Negative regulator of store-operated Ca(2+) entry (SOCE) involved in protecting cells from Ca(2+) overfilling. In response to cytosolic Ca(2+) elevation after endoplasmic reticulum Ca(2+) refilling, promotes a slow inactivation of STIM (STIM1 or STIM2)- dependent SOCE activity: possibly act by facilitating the deoligomerization of STIM to efficiently turn off ORAI when the endoplasmic reticulum lumen is filled with the appropriate Ca(2+) levels, and thus preventing the overload of the cell with excessive Ca(2+) ions.

Alternative splicing; Calcium; Calcium transport; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Ion transport; Membrane; Polymorphism; Reference proteome; Signal; Transmembrane; Transmembrane helix; Transport.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0030176; C:integral to endoplasmic reticulum membrane; IDA:UniProtKB.
GO:0015279; F:store-operated calcium channel activity; IDA:UniProtKB.
GO:2001256; P:regulation of store-operated calcium entry; IDA:UniProtKB.

IPR009567; DUF1183_TMEM66.