CPLM-019554

CPLM 1.0 - Compendium of Protein Lysine Modification

Tag

Content

CPLM ID

CPLM-019554

UniProt Accession

KI20B_HUMAN; Q96Q89; A8MXM7; O43277; Q09471; Q2KQ73; Q32NE1; Q561V3; Q58EX8; Q5T9M8; Q5T9M9; Q5T9N0; Q5T9N1; Q7KZ68; Q7Z5E0; Q7Z5E1; Q7Z6M9; Q86X82; Q9H3R8; Q9H6Q9; Q9H755; Q9NTC1; Q9UFR5

Genbank Protein ID

AB033337; AB033338; AL117496; AK024959; AK025628; AL157389; AL157400; AL157389; AL157400; AL157389; AL157400; AL157400; AL157389; AL157400; AL157389; AL157400; AL157389; AL157400; AY282406; AY282407; BC046134; BC058913; BC093089; BC108688; AL137392; U93121; AY739715; L16782

Genbank Nucleotide ID

BAB69456.1; BAB20417.1; CAB55962.1; BAB15043.1; BAB15194.1; CAI12885.1; CAI12885.1; CAI12886.1; CAI12886.1; CAI12887.1; CAI12887.1; CAI16829.1; CAI16829.1; CAI16830.1; CAI16830.1; CAI16831.1; CAI16831.1; CAI16832.1; AAP40330.1; AAP40331.1; AAH46134.1; AAH58913.1; AAH93089.1; AAI08689.1; CAB70720.1; AAB88727.1; AAW65984.1; AAC37542.1

Protein Name

Kinesin-like protein KIF20B

Protein Synonyms/Alias

Cancer/testis antigen 90; CT90; Kinesin-related motor interacting with PIN1; M-phase phosphoprotein 1; MPP1

Gene Name

KIF20B

Gene Synonyms/Alias

KRMP1; MPHOSPH1

Created Date

July 27, 2013

Organism

Homo sapiens (Human)

NCBI Taxa ID

9606

Lysine Modification

Position	Peptide	Type	References
117	AQKFSFSKVFGPATT	ubiquitination	[1, 2]
343	HQSVAFTKLNNASSR	ubiquitination	[3]
767	ITQNQRIKELINIID	ubiquitination	[1]
983	TNNVSQIKLMHTKID	ubiquitination	[1]
1202	LKLERNLKEFQEHLQ	ubiquitination	[1]
1345	QRTIQQLKEQLNNQK	ubiquitination	[1]

Reference

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]

Functional Description

Plus-end-directed motor enzyme that is required for completion of cytokinesis.

Sequence Annotation

DOMAIN 55 405 Kinesin-motor.
NP_BIND 152 159 ATP (Potential).
REGION 1560 1820 Interaction with PIN1.
MOD_RES 303 303 Phosphoserine (By similarity).
MOD_RES 488 488 Phosphoserine.
MOD_RES 1644 1644 Phosphothreonine; by CDK1.
MOD_RES 1658 1658 Phosphoserine.

Keyword

Alternative splicing; ATP-binding; Cell cycle; Cell division; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Microtubule; Mitosis; Motor protein; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome.

Sequence Source

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

Protein Length

1820 AA

Protein Sequence

MESNFNQEGV PRPSYVFSAD PIARPSEINF DGIKLDLSHE FSLVAPNTEA NSFESKDYLQ 60
VCLRIRPFTQ SEKELESEGC VHILDSQTVV LKEPQCILGR LSEKSSGQMA QKFSFSKVFG 120
PATTQKEFFQ GCIMQPVKDL LKGQSRLIFT YGLTNSGKTY TFQGTEENIG ILPRTLNVLF 180
DSLQERLYTK MNLKPHRSRE YLRLSSEQEK EEIASKSALL RQIKEVTVHN DSDDTLYGSL 240
TNSLNISEFE ESIKDYEQAN LNMANSIKFS VWVSFFEIYN EYIYDLFVPV SSKFQKRKML 300
RLSQDVKGYS FIKDLQWIQV SDSKEAYRLL KLGIKHQSVA FTKLNNASSR SHSIFTVKIL 360
QIEDSEMSRV IRVSELSLCD LAGSERTMKT QNEGERLRET GNINTSLLTL GKCINVLKNS 420
EKSKFQQHVP FRESKLTHYF QSFFNGKGKI CMIVNISQCY LAYDETLNVL KFSAIAQKVC 480
VPDTLNSSQE KLFGPVKSSQ DVSLDSNSNS KILNVKRATI SWENSLEDLM EDEDLVEELE 540
NAEETQNVET KLLDEDLDKT LEENKAFISH EEKRKLLDLI EDLKKKLINE KKEKLTLEFK 600
IREEVTQEFT QYWAQREADF KETLLQEREI LEENAERRLA IFKDLVGKCD TREEAAKDIC 660
ATKVETEETH NYVGFEDIID SLQDNVADIK KQAEIAHLYI ASLPDPQEAT ACLELKFNQI 720
KAELAKTKGE LIKTKEELKK RENESDSLIQ ELETSNKKII TQNQRIKELI NIIDQKEDTI 780
NEFQNLKSHM ENTFKCNDKA DTSSLIINNK LICNETVEVP KDSKSKICSE RKRVNENELQ 840
QDEPPAKKGS IHVSSAITED QKKSEEVRPN IAEIEDIRVL QENNEGLRAF LLTIENELKN 900
EKEEKAELNK QIVHFQQELS LSEKKNLTLS KEVQQIQSNY DIAIAELHVQ KSKNQEQEEK 960
IMKLSNEIET ATRSITNNVS QIKLMHTKID ELRTLDSVSQ ISNIDLLNLR DLSNGSEEDN 1020
LPNTQLDLLG NDYLVSKQVK EYRIQEPNRE NSFHSSIEAI WEECKEIVKA SSKKSHQIEE 1080
LEQQIEKLQA EVKGYKDENN RLKEKEHKNQ DDLLKEKETL IQQLKEELQE KNVTLDVQIQ 1140
HVVEGKRALS ELTQGVTCYK AKIKELETIL ETQKVECSHS AKLEQDILEK ESIILKLERN 1200
LKEFQEHLQD SVKNTKDLNV KELKLKEEIT QLTNNLQDMK HLLQLKEEEE ETNRQETEKL 1260
KEELSASSAR TQNLKADLQR KEEDYADLKE KLTDAKKQIK QVQKEVSVMR DEDKLLRIKI 1320
NELEKKKNQC SQELDMKQRT IQQLKEQLNN QKVEEAIQQY ERACKDLNVK EKIIEDMRMT 1380
LEEQEQTQVE QDQVLEAKLE EVERLATELE KWKEKCNDLE TKNNQRSNKE HENNTDVLGK 1440
LTNLQDELQE SEQKYNADRK KWLEEKMMLI TQAKEAENIR NKEMKKYAED RERFFKQQNE 1500
MEILTAQLTE KDSDLQKWRE ERDQLVAALE IQLKALISSN VQKDNEIEQL KRIISETSKI 1560
ETQIMDIKPK RISSADPDKL QTEPLSTSFE ISRNKIEDGS VVLDSCEVST ENDQSTRFPK 1620
PELEIQFTPL QPNKMAVKHP GCTTPVTVKI PKARKRKSNE MEEDLVKCEN KKNATPRTNL 1680
KFPISDDRNS SVKKEQKVAI RPSSKKTYSL RSQASIIGVN LATKKKEGTL QKFGDFLQHS 1740
PSILQSKAKK IIETMSSSKL SNVEASKENV SQPKRAKRKL YTSEISSPID ISGQVILMDQ 1800
KMKESDHQII KRRLRTKTAK 1820

Gene Ontology

GO:0005813; C:centrosome; IDA:UniProtKB.
GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO:0005874; C:microtubule; IEA:UniProtKB-KW.
GO:0005875; C:microtubule associated complex; IEA:InterPro.
GO:0005730; C:nucleolus; IDA:UniProtKB.
GO:0005654; C:nucleoplasm; IDA:UniProtKB.
GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO:0016887; F:ATPase activity; IDA:UniProtKB.
GO:0003777; F:microtubule motor activity; IEA:InterPro.
GO:0007050; P:cell cycle arrest; NAS:UniProtKB.
GO:0051301; P:cell division; IEA:UniProtKB-KW.
GO:0000087; P:M phase of mitotic cell cycle; TAS:ProtInc.
GO:0007018; P:microtubule-based movement; IEA:InterPro.
GO:0007067; P:mitosis; IEA:UniProtKB-KW.
GO:0007088; P:regulation of mitosis; NAS:UniProtKB.

Interpro

IPR019821; Kinesin_motor_CS.
IPR001752; Kinesin_motor_dom.
IPR027417; P-loop_NTPase.

Pfam

PF00225; Kinesin

SMART

SM00129; KISc

PROSITE

PS00411; KINESIN_MOTOR_DOMAIN1
PS50067; KINESIN_MOTOR_DOMAIN2

PRINTS

PR00380; KINESINHEAVY.