UniProt Accession

 KPYR_HUMAN; P30613; P11973 

Genbank Protein ID

 AB015983; M15465; AY316591; BC025737; S60712 

Genbank Nucleotide ID

 BAA31706.1; AAA60104.1; AAP69527.1; AAH25737.1; AAB26262.1 

Protein Name

 Pyruvate kinase PKLR 

Protein Synonyms/Alias

 Pyruvate kinase 1; Pyruvate kinase isozymes L/R; R-type/L-type pyruvate kinase; Red cell/liver pyruvate kinase 

Gene Name

 PKLR 

Gene Synonyms/Alias

 PK1; PKL 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
348	GIEIPAEKVFLAQKM	ubiquitination	[1, 2]
354	EKVFLAQKMMIGRCN	ubiquitination	[1, 2]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Plays a key role in glycolysis (By similarity). 

Sequence Annotation

 REGION 475 480 Allosteric activator binding.
 REGION 559 564 Allosteric activator binding.
 METAL 118 118 Potassium.
 METAL 120 120 Potassium.
 METAL 156 156 Potassium.
 METAL 157 157 Potassium; via carbonyl oxygen.
 METAL 315 315 Magnesium.
 METAL 339 339 Magnesium.
 BINDING 116 116 Substrate.
 BINDING 338 338 Substrate; via amide nitrogen.
 BINDING 339 339 Substrate; via amide nitrogen.
 BINDING 371 371 Substrate.
 BINDING 525 525 Allosteric activator.
 BINDING 532 532 Allosteric activator.  

Keyword

 3D-structure; Allosteric enzyme; Alternative splicing; ATP-binding; Complete proteome; Disease mutation; Glycolysis; Hereditary hemolytic anemia; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Polymorphism; Potassium; Pyruvate; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 574 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0030955; F:potassium ion binding; IEA:InterPro.
 GO:0004743; F:pyruvate kinase activity; TAS:ProtInc.
 GO:0006754; P:ATP biosynthetic process; IEA:Compara.
 GO:0032869; P:cellular response to insulin stimulus; IEA:Compara.
 GO:0031018; P:endocrine pancreas development; TAS:Reactome.
 GO:0006112; P:energy reserve metabolic process; TAS:Reactome.
 GO:0006096; P:glycolysis; TAS:Reactome.
 GO:0031325; P:positive regulation of cellular metabolic process; TAS:Reactome.
 GO:0042866; P:pyruvate biosynthetic process; IEA:Compara.
 GO:0033198; P:response to ATP; IEA:Compara.
 GO:0051591; P:response to cAMP; IEA:Compara.
 GO:0009749; P:response to glucose stimulus; IEA:Compara.
 GO:0009408; P:response to heat; IEA:Compara.
 GO:0001666; P:response to hypoxia; IEA:Compara.
 GO:0010226; P:response to lithium ion; IEA:Compara.
 GO:0007584; P:response to nutrient; IEA:Compara.
 GO:0051707; P:response to other organism; IEA:Compara.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 

Interpro

 IPR001697; Pyr_Knase.
 IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
 IPR011037; Pyrv_Knase-like_insert_dom.
 IPR015794; Pyrv_Knase_a/b.
 IPR018209; Pyrv_Knase_AS.
 IPR015793; Pyrv_Knase_brl.
 IPR015795; Pyrv_Knase_C.
 IPR015806; Pyrv_Knase_insert_dom. 

Pfam

 PF00224; PK
 PF02887; PK_C 

SMART

  

PROSITE

 PS00110; PYRUVATE_KINASE 

PRINTS

 PR01050; PYRUVTKNASE.