UniProt Accession

 FBRL_HUMAN; P22087; B5BUE8; O75259; Q6IAT5; Q9UPI6 

Genbank Protein ID

 M59849; X56597; BT006830; BT020144; CR457069; AB451384; AC006950; AC005393; CH471126; BC019260 

Genbank Nucleotide ID

 AAA52453.1; CAA39935.1; AAP35476.1; AAV38946.1; CAG33350.1; BAG70198.1; AAD15623.1; AAC28913.1; EAW56925.1; AAH19260.1 

Protein Name

 rRNA 2'-O-methyltransferase fibrillarin 

Protein Synonyms/Alias

 34 kDa nucleolar scleroderma antigen 

Gene Name

 FBL 

Gene Synonyms/Alias

 FIB1; FLRN 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
102	GVFICRGKEDALVTK	ubiquitination	[1, 2, 3]
109	KEDALVTKNLVPGES	ubiquitination	[1, 3, 4, 5, 6, 7]
121	GESVYGEKRVSISEG	ubiquitination	[1, 2, 3, 6]
131	SISEGDDKIEYRAWN	ubiquitination	[1, 3]
143	AWNPFRSKLAAAILG	ubiquitination	[3]
158	GVDQIHIKPGAKVLY	ubiquitination	[1, 3, 4, 7]
205	RDLINLAKKRTNIIP	acetylation	[8]
205	RDLINLAKKRTNIIP	ubiquitination	[1, 3]
206	DLINLAKKRTNIIPV	ubiquitination	[3]
222	EDARHPHKYRMLIAM	ubiquitination	[3]
284	AVFASEVKKMQQENM	ubiquitination	[2, 3]
285	VFASEVKKMQQENMK	ubiquitination	[3]
292	KMQQENMKPQEQLTL	ubiquitination	[1, 3, 6]
318	GVYRPPPKVKN****	ubiquitination	[4, 7]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [8] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 

Functional Description

 Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'- hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. 

Sequence Annotation

 REGION 172 173 S-adenosyl-L-methionine binding (By
 REGION 191 192 S-adenosyl-L-methionine binding (By
 REGION 216 217 S-adenosyl-L-methionine binding (By
 REGION 274 306 Helical (Potential).
 BINDING 143 143 S-adenosyl-L-methionine (By similarity).
 BINDING 236 236 S-adenosyl-L-methionine (By similarity).
 MOD_RES 8 8 Asymmetric dimethylarginine (By
 MOD_RES 15 15 Asymmetric dimethylarginine (By
 MOD_RES 21 21 Asymmetric dimethylarginine (By
 MOD_RES 24 24 Asymmetric dimethylarginine (By
 MOD_RES 27 27 Asymmetric dimethylarginine (By
 MOD_RES 124 124 Phosphoserine.  

Keyword

 3D-structure; Complete proteome; Methylation; Methyltransferase; Nucleus; Phosphoprotein; Reference proteome; Ribonucleoprotein; RNA-binding; rRNA processing; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 321 AA 

Protein Sequence

Gene Ontology

 GO:0031428; C:box C/D snoRNP complex; NAS:BHF-UCL.
 GO:0015030; C:Cajal body; IDA:BHF-UCL.
 GO:0001651; C:dense fibrillar component; IEA:Compara.
 GO:0001652; C:granular component; IEA:Compara.
 GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
 GO:0003723; F:RNA binding; TAS:ProtInc.
 GO:0006364; P:rRNA processing; TAS:ProtInc.
 GO:0016074; P:snoRNA metabolic process; IEA:Compara.
 GO:0008033; P:tRNA processing; IEA:InterPro. 

Interpro

 IPR000692; Fibrillarin.
 IPR020813; Fibrillarin_CS. 

Pfam

 PF01269; Fibrillarin 

SMART

  

PROSITE

 PS00566; FIBRILLARIN 

PRINTS

 PR00052; FIBRILLARIN.