UniProt Accession

 RFA1_YEAST; P22336; D6VPL9; P38906 

Genbank Protein ID

 X59748; M60262; S64901; L22015; BK006935 

Genbank Nucleotide ID

 CAA42420.1; AAA34994.1; AAB27889.1; AAC04960.1; DAA06989.1 

Protein Name

 Replication factor A protein 1 

Protein Synonyms/Alias

 RF-A protein 1; DNA-binding protein BUF2; Replication protein A 69 kDa DNA-binding subunit; Single-stranded DNA-binding protein 

Gene Name

 RFA1 

Gene Synonyms/Alias

 BUF2; RPA1; YAR007C; FUN3 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
427	NANFITLKQEPGMGG	acetylation	[1]
463	ENLGRSEKGDFFSVK	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Binds to single-stranded sequences participating in DNA replication in addition to those mediating transcriptional repression (URS1) and activation (CAR1). Stimulates the activity of a cognate strand exchange protein (SEP1). It cooperates with T- AG and DNA topoisomerase I to unwind template DNA containing the simian virus 40 origin of DNA replication. 

Sequence Annotation

 ZN_FING 486 508 C4-type (Potential).
 MOD_RES 178 178 Phosphoserine; by ATM or ATR.  

Keyword

 3D-structure; Complete proteome; Direct protein sequencing; DNA replication; DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 621 AA 

Protein Sequence

Gene Ontology

 GO:0000781; C:chromosome, telomeric region; IMP:SGD.
 GO:0000794; C:condensed nuclear chromosome; IDA:SGD.
 GO:0005829; C:cytosol; IDA:SGD.
 GO:0005662; C:DNA replication factor A complex; IDA:SGD.
 GO:0003690; F:double-stranded DNA binding; IDA:SGD.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0043565; F:sequence-specific DNA binding; IDA:SGD.
 GO:0003697; F:single-stranded DNA binding; IDA:SGD.
 GO:0006268; P:DNA unwinding involved in replication; IDA:SGD.
 GO:0000724; P:double-strand break repair via homologous recombination; IGI:SGD.
 GO:0045184; P:establishment of protein localization; IPI:SGD.
 GO:0030491; P:heteroduplex formation; IDA:SGD.
 GO:0006289; P:nucleotide-excision repair; IDA:SGD.
 GO:0016567; P:protein ubiquitination; IMP:SGD.
 GO:0007131; P:reciprocal meiotic recombination; IMP:SGD.
 GO:0000722; P:telomere maintenance via recombination; IGI:SGD.
 GO:0007004; P:telomere maintenance via telomerase; IPI:SGD. 

Interpro

 IPR012340; NA-bd_OB-fold.
 IPR004365; NA-bd_OB_tRNA-helicase.
 IPR013955; Rep_factor-A_C.
 IPR007199; Rep_factor-A_N.
 IPR004591; Rep_factor_Rpa1. 

Pfam

 PF04057; Rep-A_N
 PF08646; Rep_fac-A_C
 PF01336; tRNA_anti 

SMART

  

PROSITE

  

PRINTS