CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014620
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Trifunctional enzyme subunit alpha, mitochondrial 
Protein Synonyms/Alias
 TP-alpha; Long-chain enoyl-CoA hydratase; Long chain 3-hydroxyacyl-CoA dehydrogenase 
Gene Name
 Hadha 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
46THINYGVKGDVAVIRacetylation[1]
125RISQEGQKMFEKLEKacetylation[1]
129EGQKMFEKLEKSPKPacetylation[1]
166RIATKDRKTVLGVPEacetylation[1]
214IRADRAKKMGLVDQLacetylation[1]
249EVAVNFAKGLADRKVacetylation[1]
284FVRQQVYKTVEEKVKacetylation[1]
289VYKTVEEKVKKQTKGacetylation[1]
295EKVKKQTKGLYPAPLacetylation[1]
303GLYPAPLKIIDAVKTacetylation[1]
351NGQVLCKKNKFGAPQacetylation[1]
353QVLCKKNKFGAPQKTacetylation[1]
386VSVDKGLKTLLKDTTacetylation[1]
390KGLKTLLKDTTVTGLacetylation[1]
406RGQQQVFKGLNDKVKacetylation[1]
411VFKGLNDKVKKKALTacetylation[1]
415LNDKVKKKALTSFERacetylation[1]
436LIGQLDYKGFEKADMacetylation[1]
460AVKHKVLKEVESVTPacetylation[1]
505HYFSPVDKMQLLEIIacetylation[1]
519ITTDKTSKDTTASAVacetylation[1]
531SAVAVGLKQGKVIIVacetylation[1]
540GKVIIVVKDGPGFYTacetylation[1]
569LQEGVDPKKLDALTTacetylation[1]
625LLKLMVSKGFLGRKSacetylation[1]
646YQSGSKNKNLNSEIDacetylation[1]
664VNLRLPAKPEVSSDEacetylation[1]
728VDLYGAQKVVDRLRKacetylation[1]
735KVVDRLRKYESAYGTacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Bifunctional subunit. 
Sequence Annotation
 MOD_RES 129 129 N6-acetyllysine (By similarity).
 MOD_RES 295 295 N6-acetyllysine (By similarity).
 MOD_RES 303 303 N6-acetyllysine (By similarity).
 MOD_RES 326 326 N6-acetyllysine (By similarity).
 MOD_RES 350 350 N6-acetyllysine (By similarity).
 MOD_RES 406 406 N6-acetyllysine (By similarity).
 MOD_RES 505 505 N6-acetyllysine (By similarity).
 MOD_RES 540 540 N6-acetyllysine (By similarity).
 MOD_RES 569 569 N6-acetyllysine (By similarity).
 MOD_RES 644 644 N6-acetyllysine (By similarity).
 MOD_RES 728 728 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; Complete proteome; Fatty acid metabolism; Lipid metabolism; Lyase; Mitochondrion; Multifunctional enzyme; NAD; Oxidoreductase; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 763 AA 
Protein Sequence
MVASRAIGSL SRFSAFRILR SRGCICHSFT TSSALLSRTH INYGVKGDVA VIRINSPNSK 60
VNTLNKEVQS EFVEVMNEIW ANDQIRSAVL ISSKPGCFVA GADINMLASC TTPQEAARIS 120
QEGQKMFEKL EKSPKPVVAA ISGSCLGGGL ELAIACQYRI ATKDRKTVLG VPEVLLGILP 180
GAGGTQRLPK MVGVPAAFDM MLTGRNIRAD RAKKMGLVDQ LVDPLGPGIK SPEERTIEYL 240
EEVAVNFAKG LADRKVSAKQ SKGLMEKLTS YAMTIPFVRQ QVYKTVEEKV KKQTKGLYPA 300
PLKIIDAVKT GLEQGNDAGY LAESEKFGEL ALTKESKALM GLYNGQVLCK KNKFGAPQKT 360
VQQLAILGAG LMGAGIAQVS VDKGLKTLLK DTTVTGLGRG QQQVFKGLND KVKKKALTSF 420
ERDSIFSNLI GQLDYKGFEK ADMVIEAVFE DLAVKHKVLK EVESVTPEHC IFASNTSALP 480
INQIAAVSQR PEKVIGMHYF SPVDKMQLLE IITTDKTSKD TTASAVAVGL KQGKVIIVVK 540
DGPGFYTTRC LAPMMSEVIR ILQEGVDPKK LDALTTGFGF PVGAATLADE VGIDVAQHVA 600
EDLGKAFGER FGGGSVELLK LMVSKGFLGR KSGKGFYIYQ SGSKNKNLNS EIDNILVNLR 660
LPAKPEVSSD EDIQYRVITR FVNEAVLCLQ EGILATPEEG DIGAVFGLGF PPCLGGPFRF 720
VDLYGAQKVV DRLRKYESAY GTQFTPCQLL RDLANNSSKK FYQ 763 
Gene Ontology
 GO:0016507; C:mitochondrial fatty acid beta-oxidation multienzyme complex; IDA:RGD.
 GO:0005743; C:mitochondrial inner membrane; IEA:Compara.
 GO:0042645; C:mitochondrial nucleoid; IEA:Compara.
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IDA:RGD.
 GO:0003988; F:acetyl-CoA C-acyltransferase activity; IDA:RGD.
 GO:0004300; F:enoyl-CoA hydratase activity; IDA:RGD.
 GO:0000062; F:fatty-acyl-CoA binding; IDA:RGD.
 GO:0016509; F:long-chain-3-hydroxyacyl-CoA dehydrogenase activity; IDA:RGD.
 GO:0016508; F:long-chain-enoyl-CoA hydratase activity; IDA:RGD.
 GO:0051287; F:NAD binding; IDA:RGD.
 GO:0032403; F:protein complex binding; IMP:RGD.
 GO:0006635; P:fatty acid beta-oxidation; IDA:RGD.
 GO:0042493; P:response to drug; IDA:RGD.
 GO:0032868; P:response to insulin stimulus; IEA:Compara. 
Interpro
 IPR006180; 3-OHacyl-CoA_DH_CS.
 IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
 IPR006108; 3HC_DH_C.
 IPR008927; 6-PGluconate_DH_C-like.
 IPR001753; Crotonase_core_superfam.
 IPR013328; DH_multihelical.
 IPR018376; Enoyl-CoA_hyd/isom_CS.
 IPR012803; Fa_ox_alpha_mit.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF00725; 3HCDH
 PF02737; 3HCDH_N
 PF00378; ECH 
SMART
  
PROSITE
 PS00067; 3HCDH
 PS00166; ENOYL_COA_HYDRATASE 
PRINTS