CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022710
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Afadin 
Protein Synonyms/Alias
 Protein Af-6 
Gene Name
 Mllt4 
Gene Synonyms/Alias
 Af6 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
90MRMLSSPKYSLYEVHubiquitination[1]
258RIYADSLKPNIPYKTubiquitination[1]
315HSDERGAKEIILDDDubiquitination[1]
336FREWPSDKGILVFQLubiquitination[1]
383GSALPPEKLPYLVELubiquitination[1]
998EIITVTLKKQNGMGLubiquitination[1]
1027IYVKSVVKGGAADVDubiquitination[1]
1222YFTFPASKSQDRMAPubiquitination[1]
1322TPASTLTKSGPGRWKubiquitination[1]
1699QRNASYLKTQVLSPDubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton. May play a key role in the organization of epithelial structures of the embryonic ectoderm. 
Sequence Annotation
 DOMAIN 39 133 Ras-associating 1.
 DOMAIN 246 348 Ras-associating 2.
 DOMAIN 426 492 FHA.
 DOMAIN 653 908 Dilute.
 DOMAIN 1007 1093 PDZ.
 MOD_RES 216 216 Phosphoserine (By similarity).
 MOD_RES 1107 1107 Phosphoserine.
 MOD_RES 1182 1182 Phosphoserine.
 MOD_RES 1232 1232 Phosphothreonine (By similarity).
 MOD_RES 1275 1275 Phosphoserine.
 MOD_RES 1719 1719 Phosphoserine (By similarity).
 MOD_RES 1795 1795 Phosphoserine (By similarity).  
Keyword
 3D-structure; Alternative splicing; Cell adhesion; Cell junction; Coiled coil; Complete proteome; Phosphoprotein; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1820 AA 
Protein Sequence
MSAGGRDEER RKLADIIHHW NANRLDLFEI SQPTEDLEFH GVMRFYFQDK AAGNFATKCI 60
RVSSTATTQD VIETLAEKFR PDMRMLSSPK YSLYEVHVSG ERRLDIDEKP LVVQLNWNKD 120
DREGRFVLKN ENDAIPAKKA QSNGPEKQEK EGVIQNFKRT LSKKEKKEKK KKEKEALRQA 180
SDKEERPSQG DDSENSRLAA EVYKDMPETS FTRTISNPEV VMKRRRQQKL EKRMQEFRSS 240
DGRPDSGGTL RIYADSLKPN IPYKTILLST TDTADFAVAE SLEKYGLEKE NPKDYCIARV 300
MLPPGAQHSD ERGAKEIILD DDECPLQIFR EWPSDKGILV FQLKRRPPDY IPKKMKKHVE 360
GKSLKGKDRA DGSGYGSALP PEKLPYLVEL SPDGSDSRDK PKLYRLQLSV TEVGTEKFDD 420
NSIQLFGPGI QPHHCDLTNM DGVVTVTPRS MDAETYVDGQ RISETTMLQS GMRLQFGTSH 480
VFKFVDPIQD HVLSKRSVDG GLMVKGPRHK PGAVQETTFE LGGDVHSGTA LPASRSTTRL 540
DSDRVSSASS TAERGMVKPM IRLDQEQEYR RRENRTQDAT GPELILPASI EFRESSEDSF 600
LSAIINYTNS STVHFKLSPT YVLYMACRYV LSSQHRPDIS PTERTHKAIA VVNKMVSMME 660
GVIQKQKNIA GALAFWMANA SELLNFIKQD RDLSRITLDA QDVLAHLVQM AFKYLVHCLQ 720
SELNNYMPAF LDDPEENSLQ RPKIDDVLHT LTGAMSLLRR CRVNAALTIQ LFSQLFHFIN 780
MWLFNRLVTD PDSGLCSHYW GAIIRQQLGH IEAWAEKQGL ELAADCHLSR IVQATTLLTM 840
DKYVPDDIPN INSTCFKLNS LQLQALLQNY HCAPDEPFIP TDLIENVVAV AENTADELAR 900
SDGRDVQLEE DPDLQLPFLL PEDGYSCDVV RNIPNGLQEF LDPLCQRGFC RLVPHTRSPG 960
TWTIYFEGAD YESHLMRENA ELAQPLRKEP EIITVTLKKQ NGMGLSIVAA KGAGQDKLGI 1020
YVKSVVKGGA ADVDGRLAAG DQLLSVDGRS LVGLSQERAA ELMTRTSSVV TLEVAKQGAI 1080
YHGLATLLNQ PSPMMQRISD RRGSGKPRPK SEGFELYNNS AQNGSPESPQ MPWTEYSEPK 1140
KLPGDDRLMK NRADHRSSPN VANQPPSPGG KGPYTSGTAA KITSVSTGNL CTEEQSPPPR 1200
PEAYPIPTQT YTREYFTFPA SKSQDRMAPP QSQWPNYEEK PHVHTESNHS SIAIQRVTRS 1260
QEELREEKVY QLERHRVEAG MDRKCDSDMW INQSSSVESS TSSQEHLNHS SKSVTPASTL 1320
TKSGPGRWKT PAAVLPTPVA VSQPIRTDLP PPPPPPPVHY TSEFDGIPMD LPLPPPPANQ 1380
AGPQSAQVAA AEWKKREEHQ RWYEKEKARL EEERERKRRE QERKLGQMRS QTLNPASFSP 1440
LATQAKPEKP STLQRPQETV IRELQPQQQP RTIERKDLQY ITISKEELSS GDSLSPDPWK 1500
RDAREKLEKQ QQMHIVDMLS KEIHELQNKV DRTAEESDRL RKLMLEWQFQ KRLQESKQKD 1560
EDDDEEEDDD VDTMLIMQRL EAERRARMQD EERRRQQQLE EMRKREAEDR VRQEEDGRHQ 1620
EEERVKRDAE EKRRQEEGYY SRLEAERRRQ HEEAARRLLE PEEPGLSRPP LPRDYEPPSL 1680
SSAPCAPPPP PQRNASYLKT QVLSPDSLFT AKFVAYDEEE EDYGPAGPNS YSGSAGTAVG 1740
AYDAPREARE KLTRSQDADL PGSSGAPENL TFKERQRLFS QGQDVSDKVK ASRKLTELEN 1800
ELNTK 1805 
Gene Ontology
 GO:0045177; C:apical part of cell; IDA:MGI.
 GO:0005913; C:cell-cell adherens junction; IDA:MGI.
 GO:0005737; C:cytoplasm; IDA:MGI.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
 GO:0007165; P:signal transduction; IEA:InterPro. 
Interpro
 IPR018444; Dil_domain.
 IPR002710; Dilute.
 IPR000253; FHA_dom.
 IPR001478; PDZ.
 IPR000159; Ras-assoc.
 IPR008984; SMAD_FHA_domain. 
Pfam
 PF01843; DIL
 PF00498; FHA
 PF00595; PDZ
 PF00788; RA 
SMART
 SM00240; FHA
 SM00228; PDZ
 SM00314; RA 
PROSITE
 PS51126; DILUTE
 PS50006; FHA_DOMAIN
 PS50106; PDZ
 PS50200; RA 
PRINTS