CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014284
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 25-hydroxycholesterol 7-alpha-hydroxylase 
Protein Synonyms/Alias
 Cytochrome P450 7B1; Hippocampal transcript 1 protein; HCT-1; Oxysterol 7-alpha-hydroxylase 
Gene Name
 Cyp7b1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
62GMALKFFKDPLTFLKubiquitination[1]
69KDPLTFLKTLQRQHGubiquitination[1]
102FQYQYVTKNPKQLSFubiquitination[1]
105QYVTKNPKQLSFQKFubiquitination[1]
111PKQLSFQKFSSRLSAubiquitination[1]
119FSSRLSAKAFSVKKLubiquitination[1]
125AKAFSVKKLLTDDDLubiquitination[1]
169LFESQLLKITDWNTEubiquitination[1]
204KILAGNKKQIISELRubiquitination[1]
242NEESMQKKIIKCLTSubiquitination[1]
245SMQKKIIKCLTSEKVubiquitination[1]
251IKCLTSEKVAQMQGQubiquitination[1]
260AQMQGQSKIVQERQDubiquitination[1]
331LQSTGQKKGPGISVHubiquitination[1]
381MNLSLESKSFSLRKGubiquitination[1]
387SKSFSLRKGDFVALFubiquitination[1]
409PEIFDAPKEFRFDRFubiquitination[1]
434FKGGKKLKTYVMPFGubiquitination[1]
446PFGLGTSKCPGRYFAubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
  
Sequence Annotation
 METAL 447 447 Iron (heme axial ligand) (By similarity).  
Keyword
 Cholesterol metabolism; Complete proteome; Endoplasmic reticulum; Heme; Iron; Lipid metabolism; Membrane; Metal-binding; Microsome; Monooxygenase; NADP; Oxidoreductase; Reference proteome; Steroid metabolism; Sterol metabolism. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 507 AA 
Protein Sequence
MQGATTLDAA SPGPLALLGL LFAATLLLSA LFLLTRRTRR PREPPLIKGW LPYLGMALKF 60
FKDPLTFLKT LQRQHGDTFT VFLVGKYITF VLNPFQYQYV TKNPKQLSFQ KFSSRLSAKA 120
FSVKKLLTDD DLNEDVHRAY LLLQGKPLDA LLETMIQEVK ELFESQLLKI TDWNTERIFA 180
FCGSLVFEIT FATLYGKILA GNKKQIISEL RDDFFKFDDM FPYLVSDIPI QLLRNEESMQ 240
KKIIKCLTSE KVAQMQGQSK IVQERQDLLK RYYRHDDPEI GAHHLGFLWA SLANTIPAMF 300
WAMYYILRHP EAMEALRDEI DSFLQSTGQK KGPGISVHFT REQLDSLVCL ESTILEVLRL 360
CSYSSIIREV QEDMNLSLES KSFSLRKGDF VALFPPLIHN DPEIFDAPKE FRFDRFIEDG 420
KKKSTFFKGG KKLKTYVMPF GLGTSKCPGR YFAVNEMKLL LIMLLTYFDL EIIDRKPIGL 480
NHSRMFLGIQ HPDSAVSFRY KAKSWRS 507 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0043231; C:intracellular membrane-bounded organelle; TAS:UniProtKB.
 GO:0033783; F:25-hydroxycholesterol 7alpha-hydroxylase activity; IEA:EC.
 GO:0009055; F:electron carrier activity; IEA:InterPro.
 GO:0020037; F:heme binding; IEA:InterPro.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0008396; F:oxysterol 7-alpha-hydroxylase activity; IDA:UniProtKB.
 GO:0006699; P:bile acid biosynthetic process; IDA:UniProtKB.
 GO:0008203; P:cholesterol metabolic process; IEA:UniProtKB-KW.
 GO:0007586; P:digestion; TAS:UniProtKB.
 GO:0033147; P:negative regulation of intracellular estrogen receptor signaling pathway; IMP:MGI.
 GO:0050679; P:positive regulation of epithelial cell proliferation; IMP:MGI.
 GO:0060740; P:prostate gland epithelium morphogenesis; IMP:MGI. 
Interpro
 IPR001128; Cyt_P450.
 IPR024204; Cyt_P450_CYP7A1-type.
 IPR002403; Cyt_P450_E_grp-IV. 
Pfam
 PF00067; p450 
SMART
  
PROSITE
 PS00086; CYTOCHROME_P450 
PRINTS
 PR00465; EP450IV.