CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004253
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 
Protein Synonyms/Alias
 GFAT; D-fructose-6-phosphate amidotransferase; Hexosephosphate aminotransferase 
Gene Name
 GFA1 
Gene Synonyms/Alias
 YKL104C; YKL457 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
63IGKVSALKEEITKQNacetylation[1]
131LKTLLINKGYKFESDacetylation[1]
134LLINKGYKFESDTDTubiquitination[2]
206SPLLIGVKSEKKLKVacetylation[1]
237IPLKSNNKSFGLGPKubiquitination[2]
381RIDYENNKVILGGLKubiquitination[2]
549KLIPGQIKQVLKLEPacetylation[1]
553GQIKQVLKLEPRIKKacetylation[1]
560KLEPRIKKLCATELKubiquitination[2]
712DFPRNLAKSVTVE**ubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Involved in amino sugar synthesis (formation of chitin, supplies the amino sugars of asparagine-linked oligosaccharides of glycoproteins). 
Sequence Annotation
 DOMAIN 2 318 Glutamine amidotransferase type-2.
 DOMAIN 390 529 SIS 1.
 DOMAIN 562 707 SIS 2.
 ACT_SITE 2 2 For GATase activity.
 MOD_RES 253 253 Phosphoserine.
 MOD_RES 334 334 Phosphothreonine.
 MOD_RES 336 336 Phosphoserine.  
Keyword
 Aminotransferase; Complete proteome; Glutamine amidotransferase; Phosphoprotein; Reference proteome; Repeat; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 717 AA 
Protein Sequence
MCGIFGYCNY LVERSRGEII DTLVDGLQRL EYRGYDSTGI AIDGDEADST FIYKQIGKVS 60
ALKEEITKQN PNRDVTFVSH CGIAHTRWAT HGRPEQVNCH PQRSDPEDQF VVVHNGIITN 120
FRELKTLLIN KGYKFESDTD TECIAKLYLH LYNTNLQNGH DLDFHELTKL VLLELEGSYG 180
LLCKSCHYPN EVIATRKGSP LLIGVKSEKK LKVDFVDVEF PEENAGQPEI PLKSNNKSFG 240
LGPKKAREFE AGSQNANLLP IAANEFNLRH SQSRAFLSED GSPTPVEFFV SSDAASVVKH 300
TKKVLFLEDD DLAHIYDGEL HIHRSRREVG ASMTRSIQTL EMELAQIMKG PYDHFMQKEI 360
YEQPESTFNT MRGRIDYENN KVILGGLKAW LPVVRRARRL IMIACGTSYH SCLATRAIFE 420
ELSDIPVSVE LASDFLDRKC PVFRDDVCVF VSQSGETADT MLALNYCLER GALTVGIVNS 480
VGSSISRVTH CGVHINAGPE IGVASTKAYT SQYIALVMFA LSLSDDRVSK IDRRIEIIQG 540
LKLIPGQIKQ VLKLEPRIKK LCATELKDQK SLLLLGRGYQ FAAALEGALK IKEISYMHSE 600
GVLAGELKHG VLALVDENLP IIAFGTRDSL FPKVVSSIEQ VTARKGHPII ICNENDEVWA 660
QKSKSIDLQT LEVPQTVDCL QGLINIIPLQ LMSYWLAVNK GIDVDFPRNL AKSVTVE 717 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0030246; F:carbohydrate binding; IEA:InterPro.
 GO:0004360; F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity; IDA:SGD.
 GO:0034221; P:fungal-type cell wall chitin biosynthetic process; IGI:SGD.
 GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
 GO:0006048; P:UDP-N-acetylglucosamine biosynthetic process; IEA:UniProtKB-UniPathway. 
Interpro
 IPR017932; GATase_2_dom.
 IPR000583; GATase_dom.
 IPR005855; GlmS_trans.
 IPR001347; SIS. 
Pfam
 PF00310; GATase_2
 PF01380; SIS 
SMART
  
PROSITE
 PS51278; GATASE_TYPE_2
 PS51464; SIS 
PRINTS