UniProt Accession

 SAHH_RAT; P10760 

Genbank Protein ID

 M15185; U14937 

Genbank Nucleotide ID

 AAA40705.1; AAA92043.1 

Protein Name

 Adenosylhomocysteinase 

Protein Synonyms/Alias

 AdoHcyase; S-adenosyl-L-homocysteine hydrolase 

Gene Name

 Ahcy 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
4	****MADKLPYKVAD	acetylation	[1]
8	MADKLPYKVADIGLA	acetylation	[1]
43	REMYSASKPLKGARI	acetylation	[1]
166	TGVHNLYKMMANGIL	acetylation	[1]
204	ESLIDGIKRATDVMI	acetylation	[1]
318	LNENAVEKVNIKPQV	acetylation	[1]
322	AVEKVNIKPQVDRYL	acetylation	[1]
389	GVHFLPKKLDEAVAE	ubiquitination	[2]
401	VAEAHLGKLNVKLTK	acetylation	[1]
405	HLGKLNVKLTKLTEK	acetylation	[1]
412	KLTKLTEKQAQYLGM	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 

Functional Description

 Adenosylhomocysteine is a competitive inhibitor of S- adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. 

Sequence Annotation

 NP_BIND 157 159 NAD.
 NP_BIND 222 227 NAD.
 NP_BIND 299 301 NAD.
 NP_BIND 426 430 NAD.
 BINDING 57 57 Substrate.
 BINDING 131 131 Substrate.
 BINDING 156 156 Substrate.
 BINDING 186 186 Substrate.
 BINDING 190 190 Substrate.
 BINDING 243 243 NAD.
 BINDING 248 248 NAD.
 BINDING 346 346 NAD.
 BINDING 353 353 NAD.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; NAD; One-carbon metabolism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 432 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:RGD.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0043005; C:neuron projection; IDA:RGD.
 GO:0005634; C:nucleus; IDA:RGD.
 GO:0004013; F:adenosylhomocysteinase activity; IDA:RGD.
 GO:0030554; F:adenyl nucleotide binding; IDA:RGD.
 GO:0042802; F:identical protein binding; IDA:RGD.
 GO:0051287; F:NAD binding; IDA:RGD.
 GO:0002439; P:chronic inflammatory response to antigenic stimulus; IMP:RGD.
 GO:0042745; P:circadian sleep/wake cycle; IDA:RGD.
 GO:0071268; P:homocysteine biosynthetic process; IMP:RGD.
 GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
 GO:0001666; P:response to hypoxia; IEP:RGD.
 GO:0007584; P:response to nutrient; IDA:RGD.
 GO:0019510; P:S-adenosylhomocysteine catabolic process; IDA:RGD. 

Interpro

 IPR000043; Adenosylhomocysteinase.
 IPR015878; Ado_hCys_hydrolase_NAD-bd.
 IPR016040; NAD(P)-bd_dom.
 IPR020082; S-Ado-L-homoCys_hydrolase_CS. 

Pfam

 PF05221; AdoHcyase
 PF00670; AdoHcyase_NAD 

SMART

 SM00996; AdoHcyase
 SM00997; AdoHcyase_NAD 

PROSITE

 PS00738; ADOHCYASE_1
 PS00739; ADOHCYASE_2 

PRINTS