UniProt Accession

 RPN8_YEAST; Q08723; D6W2W2; Q6Q5I7 

Genbank Protein ID

 Z75169; AY558039; BK006948 

Genbank Nucleotide ID

 CAA99483.1; AAS56365.1; DAA11028.1 

Protein Name

 26S proteasome regulatory subunit RPN8 

Protein Synonyms/Alias

  

Gene Name

 RPN8 

Gene Synonyms/Alias

 YOR261C; O5360 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
105	KLRASDLKINELFKK	ubiquitination	[1]
112	KINELFKKYTQNNPL	ubiquitination	[1]
144	YVAIEQVKDDGTSTE	ubiquitination	[1]
195	IRLTNQLKSLKGLQS	ubiquitination	[1]
198	TNQLKSLKGLQSKLK	ubiquitination	[1]
205	KGLQSKLKDVVEYLD	ubiquitination	[1]
213	DVVEYLDKVINKELP	acetylation	[2]
217	YLDKVINKELPINHT	acetylation	[2]
263	NISNNLQKALTVKTN	ubiquitination	[1]
295	FDDLIENKIQNKKIQ	ubiquitination	[1]
324	SESESGDKEATAPLI	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. 

Sequence Annotation

 MOD_RES 2 2 N-acetylserine.
 MOD_RES 314 314 Phosphoserine.
 MOD_RES 317 317 Phosphoserine.
 MOD_RES 319 319 Phosphoserine.
 MOD_RES 327 327 Phosphothreonine.  

Keyword

 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Phosphoprotein; Proteasome; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 338 AA 

Protein Sequence

Gene Ontology

 GO:0008541; C:proteasome regulatory particle, lid subcomplex; IDA:SGD.
 GO:0034515; C:proteasome storage granule; IDA:SGD.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; TAS:SGD. 

Interpro

 IPR000555; JAB1_Mov34_MPN_PAD1.
 IPR024969; Rpn11/EIF3F_C. 

Pfam

 PF01398; JAB
 PF13012; MitMem_reg 

SMART

 SM00232; JAB_MPN 

PROSITE

  

PRINTS