UniProt Accession

 DHE2_BACSU; P39633; Q53548; Q5W7E9 

Genbank Protein ID

 X73124; AB194695; AL009126; S79622 

Genbank Nucleotide ID

 CAA51631.1; BAD69594.1; CAB15806.2 

Protein Name

 Catabolic NAD-specific glutamate dehydrogenase RocG 

Protein Synonyms/Alias

 NAD-GDH; Glutamate dehydrogenase; GlutDH; Trigger enzyme RocG 

Gene Name

 rocG 

Gene Synonyms/Alias

 gudA; yweB; BSU37790; ipa-75d 

Created Date

 July 27, 2013 

Organism

 Bacillus subtilis (strain 168) 

NCBI Taxa ID

 224308 

Lysine Modification

Position	Peptide	Type	References
26	LSTQTIIKEALRKLG	acetylation	[1]

Reference

 [1] The acetylproteome of Gram-positive model bacterium Bacillus subtilis.
 Kim D, Yu BJ, Kim JA, Lee YJ, Choi SG, Kang S, Pan JG.
 Proteomics. 2013 May;13(10-11):1726-36. [PMID: 23468065] 

Functional Description

 Devoted to catabolic function of glutamate (and other amino acids of the glutamate family) utilization as sole nitrogen source. It is not involved in anabolic function of glutamate biosynthesis since B.subtilis possesses only one route of glutamate biosynthesis from ammonia, catalyzed by glutamate synthase. RocG is unable to utilize glutamate or glutamine as sole carbon source and to synthesize glutamate, but it is involved in the utilization of arginine, and proline as carbon or nitrogen source. The catabolic RocG is essential for controlling gltAB expression via an inhibitory interactions with the transcriptional regulator GltC in response to the availability of sugars. 

Sequence Annotation

 ACT_SITE 116 116 Proton donor (By similarity).
 BINDING 80 80 Substrate (By similarity).
 BINDING 104 104 Substrate (By similarity).
 BINDING 200 200 NAD (By similarity).
 BINDING 231 231 NAD (By similarity).
 BINDING 358 358 Substrate (By similarity).  

Keyword

 3D-structure; Complete proteome; Direct protein sequencing; NAD; Oxidoreductase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 424 AA 

Protein Sequence

Gene Ontology

 GO:0004352; F:glutamate dehydrogenase (NAD+) activity; IDA:UniProtKB.
 GO:0006520; P:cellular amino acid metabolic process; IMP:UniProtKB. 

Interpro

 IPR006095; Glu/Leu/Phe/Val_DH.
 IPR006096; Glu/Leu/Phe/Val_DH_C.
 IPR006097; Glu/Leu/Phe/Val_DH_dimer_dom.
 IPR014362; Glu_DH.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF00208; ELFV_dehydrog
 PF02812; ELFV_dehydrog_N 

SMART

 SM00839; ELFV_dehydrog 

PROSITE

 PS00074; GLFV_DEHYDROGENASE 

PRINTS

 PR00082; GLFDHDRGNASE.