CPLM-022988

Genbank Nucleotide ID

Mitotic spindle assembly checkpoint protein MAD2B

Protein Synonyms/Alias

Mitotic arrest deficient 2-like protein 2; MAD2-like protein 2; REV7 homolog; hREV7

Homo sapiens (Human)

Position	Peptide	Type	References
44	YPVGIFQKRKKYNVP	ubiquitination	[1, 2]
90	VVVVILDKEHRPVEK	ubiquitination	[1]
162	AATRNMEKIQVIKDF	ubiquitination	[2]
167	MEKIQVIKDFPWILA	ubiquitination	[2]
190	DPRLIPLKTMTSDIL	ubiquitination	[1, 2]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]

Functional Description

Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation.

DOMAIN 13 203 HORMA.
REGION 21 155 Mediates interaction with REV1 and REV3L
REGION 150 211 Mediates interaction with ipaB.

3D-structure; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; DNA damage; Mitosis; Nucleus; Reference proteome; Transcription; Transcription regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO:0005654; C:nucleoplasm; TAS:Reactome.
GO:0005819; C:spindle; IDA:UniProtKB.
GO:0016035; C:zeta DNA polymerase complex; IDA:UniProtKB.
GO:0008432; F:JUN kinase binding; IDA:UniProtKB.
GO:0007015; P:actin filament organization; IMP:BHF-UCL.
GO:0051301; P:cell division; IEA:UniProtKB-KW.
GO:0042772; P:DNA damage response, signal transduction resulting in transcription; IDA:UniProtKB.
GO:0006302; P:double-strand break repair; IGI:UniProtKB.
GO:0007067; P:mitosis; IEA:UniProtKB-KW.
GO:0007094; P:mitotic spindle assembly checkpoint; TAS:ProtInc.
GO:0090090; P:negative regulation of canonical Wnt receptor signaling pathway; IMP:BHF-UCL.
GO:2000048; P:negative regulation of cell-cell adhesion mediated by cadherin; IMP:BHF-UCL.
GO:0010719; P:negative regulation of epithelial to mesenchymal transition; IMP:BHF-UCL.
GO:0060564; P:negative regulation of mitotic anaphase-promoting complex activity; IDA:UniProtKB.
GO:0043433; P:negative regulation of sequence-specific DNA binding transcription factor activity; IDA:BHF-UCL.
GO:0010944; P:negative regulation of transcription by competitive promoter binding; IMP:BHF-UCL.
GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:BHF-UCL.
GO:2000678; P:negative regulation of transcription regulatory region DNA binding; IMP:BHF-UCL.
GO:0033138; P:positive regulation of peptidyl-serine phosphorylation; IDA:UniProtKB.
GO:0045893; P:positive regulation of transcription, DNA-dependent; IMP:UniProtKB.
GO:0001558; P:regulation of cell growth; IGI:UniProtKB.
GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.

IPR003511; HORMA_DNA-bd.