UniProt Accession

 SNX9_MOUSE; Q91VH2 

Genbank Protein ID

 BC014814 

Genbank Nucleotide ID

 AAH14814.1 

Protein Name

 Sorting nexin-9 

Protein Synonyms/Alias

  

Gene Name

 Snx9 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
179	KSSSPYFKDSEPAEA	ubiquitination	[1]
207	SMKLPLNKFPGFAKP	ubiquitination	[1]
223	MEQYLLAKQLAKPKE	ubiquitination	[1]
267	GSKMYGLKSYIEYQL	ubiquitination	[1]
288	RSVNHRYKHFDWLYE	acetylation	[2, 3]
407	DAVGKFTKAMDDGVK	acetylation	[2]
425	TVGQEHWKRCTGPLP	ubiquitination	[1]
511	PDIIGAHKGAIEKVK	ubiquitination	[1]
528	DKLVATSKITPQDKQ	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 

Functional Description

 May be involved in several stages of intracellular trafficking. Plays a role in endocytosis via clathrin-coated pits, but also clathrin-independent, actin-dependent fluid-phase endocytosis. Plays a role in macropinocytosis. Promotes internalization of TNFR. Promotes degradation of EGFR after EGF signaling. Stimulates the GTPase activity of DNM1. Promotes DNM1 oligomerization. Promotes activation of the Arp2/3 complex by WASL, and thereby plays a role in the reorganization of the F- actin cytoskeleton. Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation. Has lower affinity for membranes enriched in phosphatidylinositol 3-phosphate (By similarity). 

Sequence Annotation

 DOMAIN 1 62 SH3.
 DOMAIN 250 360 PX.
 DOMAIN 392 595 BAR.
 REGION 201 213 Critical for tubulation activity (By
 BINDING 286 286 Phosphatidylinositol 4,5-bisphosphate (By
 BINDING 288 288 Phosphatidylinositol 4,5-bisphosphate (By
 BINDING 313 313 Phosphatidylinositol 4,5-bisphosphate (By
 BINDING 327 327 Phosphatidylinositol 4,5-bisphosphate (By
 MOD_RES 175 175 Phosphoserine.
 MOD_RES 288 288 N6-acetyllysine (By similarity).
 MOD_RES 536 536 Phosphothreonine.  

Keyword

 3D-structure; Acetylation; Cell membrane; Cell projection; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Endocytosis; Golgi apparatus; Lipid-binding; Membrane; Phosphoprotein; Protein transport; Reference proteome; SH3 domain; Transport; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 595 AA 

Protein Sequence

Gene Ontology

 GO:0030136; C:clathrin-coated vesicle; IEA:UniProtKB-SubCell.
 GO:0030659; C:cytoplasmic vesicle membrane; ISS:UniProtKB.
 GO:0031234; C:extrinsic to internal side of plasma membrane; ISS:UniProtKB.
 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0001726; C:ruffle; IEA:UniProtKB-SubCell.
 GO:0005802; C:trans-Golgi network; ISS:UniProtKB.
 GO:0005545; F:1-phosphatidylinositol binding; ISS:UniProtKB.
 GO:0007154; P:cell communication; IEA:InterPro.
 GO:0006886; P:intracellular protein transport; IMP:MGI.
 GO:0060988; P:lipid tube assembly; ISS:UniProtKB.
 GO:0043547; P:positive regulation of GTPase activity; ISS:UniProtKB.
 GO:0032461; P:positive regulation of protein oligomerization; ISS:UniProtKB.
 GO:0006898; P:receptor-mediated endocytosis; ISS:UniProtKB. 

Interpro

 IPR001683; Phox.
 IPR001452; SH3_domain.
 IPR014536; Snx9.
 IPR019497; Sorting_nexin_WASP-bd-dom. 

Pfam

 PF10456; BAR_3_WASP_bdg
 PF00787; PX
 PF00018; SH3_1 

SMART

 SM00312; PX
 SM00326; SH3 

PROSITE

 PS51021; BAR
 PS50195; PX
 PS50002; SH3 

PRINTS