UniProt Accession

 S10A4_HUMAN; P26447; A8K7R8; D3DV46; Q6ICP8 

Genbank Protein ID

 M80563; Z18950; Z33457; CR450345; AK292083; BX470102; CH471121; CH471121; CH471121; CH471121; CH471121; CH471121; BC000838; BC016300 

Genbank Nucleotide ID

 AAA51920.1; CAA79474.1; CAA83880.1; CAG29341.1; BAF84772.1; CAI14754.1; EAW53310.1; EAW53311.1; EAW53312.1; EAW53313.1; EAW53314.1; EAW53315.1; AAH00838.1; AAH16300.1 

Protein Name

 Protein S100-A4 

Protein Synonyms/Alias

 Calvasculin; Metastasin; Placental calcium-binding protein; Protein Mts1; S100 calcium-binding protein A4 

Gene Name

 S100A4 

Gene Synonyms/Alias

 CAPL; MTS1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
7	*MACPLEKALDVMVS	acetylation	[1]
18	VMVSTFHKYSGKEGD	acetylation	[1]
22	TFHKYSGKEGDKFKL	sumoylation	[2]
35	KLNKSELKELLTREL	acetylation	[1]
35	KLNKSELKELLTREL	ubiquitination	[3, 4]
48	ELPSFLGKRTDEAAF	ubiquitination	[3, 4, 5, 6]
57	TDEAAFQKLMSNLDS	ubiquitination	[3, 4, 5, 7, 8]
96	FFEGFPDKQPRKK**	sumoylation	[2]

Reference

 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Sumoylation and nuclear translocation of S100A4 regulate IL-1beta-mediated production of matrix metalloproteinase-13.
 Miranda KJ, Loeser RF, Yammani RR.
 J Biol Chem. 2010 Oct 8;285(41):31517-24. [PMID: 20685652]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

  

Sequence Annotation

 DOMAIN 12 47 EF-hand 1.
 DOMAIN 50 85 EF-hand 2.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 7 7 N6-acetyllysine.
 MOD_RES 35 35 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Calcium; Complete proteome; Direct protein sequencing; Metal-binding; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 101 AA 

Protein Sequence

Gene Ontology

 GO:0043005; C:neuron projection; IEA:Compara.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:UniProtKB.
 GO:0005509; F:calcium ion binding; TAS:ProtInc.
 GO:0001837; P:epithelial to mesenchymal transition; TAS:HGNC.
 GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB cascade; IDA:UniProtKB. 

Interpro

 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR001751; S100/CaBP-9k_CS.
 IPR013787; S100_Ca-bd_sub. 

Pfam

 PF01023; S_100 

SMART

  

PROSITE

 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS00303; S100_CABP 

PRINTS