CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022406
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 RNA polymerase I-specific transcription initiation factor RRN3 
Protein Synonyms/Alias
 Transcription initiation factor IA; TIF-IA 
Gene Name
 RRN3 
Gene Synonyms/Alias
 TIFIA 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
22AASSSAVKKLGASRTubiquitination[1, 2]
62TVTEVLLKYKKGETNubiquitination[1, 2]
65EVLLKYKKGETNDFEubiquitination[2]
253LIIEKLLKLDVNASRubiquitination[1]
415GSFLARAKFIPLITVubiquitination[2]
480QLLSGNLKEGLQYLQubiquitination[1]
568PFDPCVLKRSKKFIDubiquitination[2]
610DEDDDFLKGEVPQNDubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Required for efficient transcription initiation by RNA polymerase I. Required for the formation of the competent preinitiation complex (PIC). Dissociates from pol I as a consequence of transcription. In vitro, cannot activate transcription in a subsequent transcription reaction (By similarity). 
Sequence Annotation
 REGION 500 651 Interaction with TWISTNB.
 REGION 557 651 Interaction with EIF3L.
 MOD_RES 170 170 Phosphoserine.
 MOD_RES 172 172 Phosphoserine.
 MOD_RES 200 200 Phosphothreonine; by MAPK9.
 MOD_RES 640 640 Phosphoserine.  
Keyword
 Complete proteome; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 651 AA 
Protein Sequence
MAAPLLHTRL PGDAAASSSA VKKLGASRTG ISNMRALEND FFNSPPRKTV RFGGTVTEVL 60
LKYKKGETND FELLKNQLLD PDIKDDQIIN WLLEFRSSIM YLTKDFEQLI SIILRLPWLN 120
RSQTVVEEYL AFLGNLVSAQ TVFLRPCLSM IASHFVPPRV IIKEGDVDVS DSDDEDDNLP 180
ANFDTCHRAL QIIARYVPST PWFLMPILVE KFPFVRKSER TLECYVHNLL RISVYFPTLR 240
HEILELIIEK LLKLDVNASR QGIEDAEETA TQTCGGTDST EGLFNMDEDE ETEHETKAGP 300
ERLDQMVHPV AERLDILMSL VLSYMKDVCY VDGKVDNGKT KDLYRDLINI FDKLLLPTHA 360
SCHVQFFMFY LCSFKLGFAE AFLEHLWKKL QDPSNPAIIR QAAGNYIGSF LARAKFIPLI 420
TVKSCLDLLV NWLHIYLNNQ DSGTKAFCDV ALHGPFYSAC QAVFYTFVFR HKQLLSGNLK 480
EGLQYLQSLN FERIVMSQLN PLKICLPSVV NFFAAITNKY QLVFCYTIIE RNNRQMLPVI 540
RSTAGGDSVQ ICTNPLDTFF PFDPCVLKRS KKFIDPIYQV WEDMSAEELQ EFKKPMKKDI 600
VEDEDDDFLK GEVPQNDTVI GITPSSFDTH FRSPSSSVGS PPVLYMQPSP L 651 
Gene Ontology
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0008283; P:cell proliferation; IEA:Compara.
 GO:0007028; P:cytoplasm organization; IEA:Compara.
 GO:0048872; P:homeostasis of number of cells; IEA:Compara.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0043066; P:negative regulation of apoptotic process; IEA:Compara.
 GO:0007000; P:nucleolus organization; IEA:Compara.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IEA:Compara.
 GO:2000142; P:regulation of DNA-dependent transcription, initiation; IEA:Compara.
 GO:0042254; P:ribosome biogenesis; IEA:Compara.
 GO:0006361; P:transcription initiation from RNA polymerase I promoter; TAS:Reactome. 
Interpro
 IPR007991; RNA_pol_I_trans_ini_fac_RRN3. 
Pfam
 PF05327; RRN3 
SMART
  
PROSITE
  
PRINTS