CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002147
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tropomyosin alpha-1 chain 
Protein Synonyms/Alias
 Alpha-tropomyosin; Tropomyosin-1 
Gene Name
 Tpm1 
Gene Synonyms/Alias
 Alpha-tm; Tpma 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
37KAAEDRSKQLEDELVubiquitination[1]
59GTEDELDKYSEALKDubiquitination[1]
77KLELAEKKATDAEADacetylation[2]
112RLATALQKLEEAEKAubiquitination[3]
205KTVTNNLKSLEAQAEubiquitination[3]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [3] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
 Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. 
Sequence Annotation
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 283 283 Phosphoserine; by DAPK1 (By similarity).
 CROSSLNK 77 77 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Acetylation; Actin-binding; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Isopeptide bond; Muscle protein; Phosphoprotein; Reference proteome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 284 AA 
Protein Sequence
MDAIKKKMQM LKLDKENALD RAEQAEADKK AAEDRSKQLE DELVSLQKKL KGTEDELDKY 60
SEALKDAQEK LELAEKKATD AEADVASLNR RIQLVEEELD RAQERLATAL QKLEEAEKAA 120
DESERGMKVI ESRAQKDEEK MEIQEIQLKE AKHIAEDADR KYEEVARKLV IIESDLERAE 180
ERAELSEGKC AELEEELKTV TNNLKSLEAQ AEKYSQKEDK YEEEIKVLSD KLKEAETRAE 240
FAERSVTKLE KSIDDLEDEL YAQKLKYKAI SEELDHALND MTSI 284 
Gene Ontology
 GO:0032059; C:bleb; IEA:Compara.
 GO:0031941; C:filamentous actin; IEA:Compara.
 GO:0030016; C:myofibril; IEA:Compara.
 GO:0032587; C:ruffle membrane; IEA:Compara.
 GO:0001725; C:stress fiber; IEA:Compara.
 GO:0060048; P:cardiac muscle contraction; IEA:Compara.
 GO:0034614; P:cellular response to reactive oxygen species; IEA:Compara.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0030049; P:muscle filament sliding; IDA:BHF-UCL.
 GO:0030336; P:negative regulation of cell migration; IGI:BHF-UCL.
 GO:0032781; P:positive regulation of ATPase activity; IDA:BHF-UCL.
 GO:0045785; P:positive regulation of cell adhesion; IGI:BHF-UCL.
 GO:0003065; P:positive regulation of heart rate by epinephrine; IEA:Compara.
 GO:0051496; P:positive regulation of stress fiber assembly; IGI:BHF-UCL.
 GO:0031529; P:ruffle organization; IGI:BHF-UCL.
 GO:0045214; P:sarcomere organization; IEA:Compara.
 GO:0055010; P:ventricular cardiac muscle tissue morphogenesis; IEA:Compara.
 GO:0042060; P:wound healing; IGI:BHF-UCL. 
Interpro
 IPR000533; Tropomyosin. 
Pfam
 PF00261; Tropomyosin 
SMART
  
PROSITE
 PS00326; TROPOMYOSIN 
PRINTS
 PR00194; TROPOMYOSIN.