CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011927
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mitogen-activated protein kinase kinase kinase 12 
Protein Synonyms/Alias
 Dual leucine zipper bearing kinase; DLK; Leucine-zipper protein kinase; ZPK; MAPK-upstream kinase; MUK; Mixed lineage kinase 
Gene Name
 MAP3K12 
Gene Synonyms/Alias
 ZPK 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
163DLKETDIKHLRKLKHubiquitination[1]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 May be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro. 
Sequence Annotation
 DOMAIN 125 366 Protein kinase.
 NP_BIND 131 139 ATP (By similarity).
 REGION 390 411 Leucine-zipper 1.
 REGION 443 464 Leucine-zipper 2.
 ACT_SITE 236 236 Proton acceptor (By similarity).
 BINDING 152 152 ATP (By similarity).  
Keyword
 Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Kinase; Magnesium; Membrane; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 859 AA 
Protein Sequence
MACLHETRTP SPSFGGFVST LSEASMRKLD PDTSDCTPEK DLTPTHVLQL HEQDAGGPGG 60
AAGSPESRAS RVRADEVRLQ CQSGSGFLEG LFGCLRPVWT MIGKAYSTEH KQQQEDLWEV 120
PFEEILDLQW VGSGAQGAVF LGRFHGEEVA VKKVRDLKET DIKHLRKLKH PNIITFKGVC 180
TQAPCYCILM EFCAQGQLYE VLRAGRPVTP SLLVDWSMGI AGGMNYLHLH KIIHRDLKSP 240
NMLITYDDVV KISDFGTSKE LSDKSTKMSF AGTVAWMAPE VIRNEPVSEK VDIWSFGVVL 300
WELLTGEIPY KDVDSSAIIW GVGSNSLHLP VPSSCPDGFK ILLRQCWNSK PRNRPSFRQI 360
LLHLDIASAD VLSTPQETYF KSQAEWREEV KLHFEKIKSE GTCLHRLEEE LVMRRREELR 420
HALDIREHYE RKLERANNLY MELNALMLQL ELKERELLRR EQALERRCPG LLKPHPSRGL 480
LHGNTMEKLI KKRNVPQKLS PHSKRPDILK TESLLPKLDA ALSGVGLPGC PKGPPSPGRS 540
RRGKTRHRKA SAKGSCGDLP GLRTAVPPHE PGGPGSPGGL GGGPSAWEAC PPALRGLHHD 600
LLLRKMSSSS PDLLSAALGS RGRGATGGAG DPGSPPPARG DTPPSEGSAP GSTSPDSPGG 660
AKGEPPPPVG PGEGVGLLGT GREGTSGRGG SRAGSQHLTP AALLYRAAVT RSQKRGISSE 720
EEEGEVDSEV ELTSSQRWPQ SLNMRQSLST FSSENPSDGE EGTASEPSPS GTPEVGSTNT 780
DERPDERSDD MCSQGSEIPL DPPPSEVIPG PEPSSLPIPH QELLRERGPP NSEDSDCDST 840
ELDNSNSVDA LRPPASLPP 859 
Gene Ontology
 GO:0005829; C:cytosol; ISS:UniProtKB.
 GO:0030426; C:growth cone; IEA:Compara.
 GO:0005886; C:plasma membrane; ISS:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004709; F:MAP kinase kinase kinase activity; IEA:EC.
 GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
 GO:0004674; F:protein serine/threonine kinase activity; ISS:UniProtKB.
 GO:0016572; P:histone phosphorylation; ISS:UniProtKB.
 GO:0007254; P:JNK cascade; ISS:UniProtKB.
 GO:0018105; P:peptidyl-serine phosphorylation; ISS:UniProtKB.
 GO:0018107; P:peptidyl-threonine phosphorylation; ISS:UniProtKB.
 GO:0046777; P:protein autophosphorylation; ISS:UniProtKB. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR017419; MAP3K12_MAP3K13.
 IPR027257; MAPKKK12.
 IPR000719; Prot_kinase_cat_dom.
 IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF07714; Pkinase_Tyr 
SMART
  
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS
 PR00109; TYRKINASE.