CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004066
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 N-terminal acetyltransferase A complex subunit NAT1 
Protein Synonyms/Alias
 NatA complex subunit NAT1; Amino-terminal, alpha-amino, acetyltransferase 1 
Gene Name
 NAT1 
Gene Synonyms/Alias
 AAA1; YDL040C; D2720 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
41KQYKKSLKLLDAILKacetylation[1]
276KRNPDNFKYYKLLEVacetylation[1]
626HDDRLKRKSDSLDENubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Non-catalytic component of the NatA N-terminal acetyltransferase, which catalyzes acetylation of proteins beginning with Met-Ser, Met-Gly and Met-Ala. N-acetylation plays a role in normal eukaryotic translation and processing, protect against proteolytic degradation and protein turnover. NAT1 anchors ARD1 and NAT5 to the ribosome and may present the N termini of nascent polypeptides for acetylation. 
Sequence Annotation
 REPEAT 20 53 TPR 1.
 REPEAT 54 87 TPR 2.
 REPEAT 91 124 TPR 3.
 REPEAT 126 162 TPR 4.
 REPEAT 241 274 TPR 5.
 REPEAT 384 417 TPR 6.
 REPEAT 452 485 TPR 7.
 REPEAT 728 761 TPR 8.
 MOD_RES 2 2 N-acetylserine (Potential).
 MOD_RES 674 674 Phosphoserine.  
Keyword
 Acetylation; Coiled coil; Complete proteome; Cytoplasm; Direct protein sequencing; Phosphoprotein; Reference proteome; Repeat; TPR repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 854 AA 
Protein Sequence
MSRKRSTKPK PAAKIALKKE NDQFLEALKL YEGKQYKKSL KLLDAILKKD GSHVDSLALK 60
GLDLYSVGEK DDAASYVANA IRKIEGASAS PICCHVLGIY MRNTKEYKES IKWFTAALNN 120
GSTNKQIYRD LATLQSQIGD FKNALVSRKK YWEAFLGYRA NWTSLAVAQD VNGERQQAIN 180
TLSQFEKLAE GKISDSEKYE HSECLMYKND IMYKAASDNQ DKLQNVLKHL NDIEPCVFDK 240
FGLLERKATI YMKLGQLKDA SIVYRTLIKR NPDNFKYYKL LEVSLGIQGD NKLKKALYGK 300
LEQFYPRCEP PKFIPLTFLQ DKEELSKKLR EYVLPQLERG VPATFSNVKP LYQRRKSKVS 360
PLLEKIVLDY LSGLDPTQDP IPFIWTNYYL SQHFLFLKDF PKAQEYIDAA LDHTPTLVEF 420
YILKARILKH LGLMDTAAGI LEEGRQLDLQ DRFINCKTVK YFLRANNIDK AVEVASLFTK 480
NDDSVNGIKD LHLVEASWFI VEQAEAYYRL YLDRKKKLDD LASLKKEVES DKSEQIANDI 540
KENQWLVRKY KGLALKRFNA IPKFYKQFED DQLDFHSYCM RKGTPRAYLE MLEWGKALYT 600
KPMYVRAMKE ASKLYFQMHD DRLKRKSDSL DENSDEIQNN GQNSSSQKKK AKKEAAAMNK 660
RKETEAKSVA AYPSDQDNDV FGEKLIETST PMEDFATEFY NNYSMQVRED ERDYILDFEF 720
NYRIGKLALC FASLNKFAKR FGTTSGLFGS MAIVLLHATR NDTPFDPILK KVVTKSLEKE 780
YSENFPLNEI SNNSFDWLNF YQEKFGKNDI NGLLFLYRYR DDVPIGSSNL KEMIISSLSP 840
LEPHSQNEIL QYYL 854 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0031415; C:NatA complex; IDA:SGD.
 GO:0006474; P:N-terminal protein amino acid acetylation; IMP:SGD. 
Interpro
 IPR021183; NatA_aux_su.
 IPR011990; TPR-like_helical.
 IPR013105; TPR_2. 
Pfam
 PF12569; NARP1
 PF07719; TPR_2 
SMART
  
PROSITE
 PS50005; TPR
 PS50293; TPR_REGION 
PRINTS