| Tag | Content |
|---|
| CPLM ID | CPLM-014586 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Eukaryotic translation initiation factor 3 subunit E |
Protein Synonyms/Alias | eIF3e; Eukaryotic translation initiation factor 3 subunit 6; eIF-3 p48 |
Gene Name | Eif3e |
Gene Synonyms/Alias | Eif3s6; Int6 |
Created Date | July 27, 2013 |
Organism | Rattus norvegicus (Rat) |
NCBI Taxa ID | 10116 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 275 | RKRRQVLKDLVKVIQ | acetylation | [1] |
|
Reference | [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns. Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV. Cell Rep. 2012 Aug 30;2(2):419-31. [ PMID: 22902405] |
Functional Description | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense- mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins (By similarity). |
Sequence Annotation | DOMAIN 290 395 PCI.
REGION 4 128 Sufficient for interaction with EPAS1 (By
REGION 9 195 Sufficient for interaction with TRIM27
REGION 351 445 Sufficient for interaction with MCM7 (By
MOD_RES 2 2 N-acetylalanine (By similarity).
MOD_RES 275 275 N6-acetyllysine (By similarity).
MOD_RES 399 399 Phosphoserine (By similarity).
MOD_RES 439 439 Phosphothreonine (By similarity).
MOD_RES 445 445 Phosphotyrosine (By similarity). |
Keyword | Acetylation; Complete proteome; Cytoplasm; Initiation factor; Nucleus; Phosphoprotein; Protein biosynthesis; Reference proteome. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 445 AA |
Protein Sequence | MAEYDLTTRI AHFLDRHLVF PLLEFLSVKE IYNEKELLQG KLDLLSDTNM VDFAMDVYKN 60
LYSDDIPHAL REKRTTVVAQ LKQLQAETEP IVKMFEDPET TRQMQSTRDG RMLFDYLADK 120
HGFRQEYLDT LYRYAKFQYE CGNYSGAAEY LYFFRVLVPA TDRNALSSLW GKLASEILMQ 180
NWDAAMEDLT RLKETIDNNS VSSPLQSLQQ RTWLIHWSLF VFFNHPKGRD NIIDLFLYQP 240
QYLNAIQTMC PHILRYLTTA VITNKDVRKR RQVLKDLVKV IQQESYTYKD PITEFVECLY 300
VNFDFDGAQK KLRECESVLV NDFFLVACLE DFIENARLFI FETFCRIHQC ISINMLADKL 360
NMTPEEAERW IVNLIRNARL DAKIDSKLGH VVMGNNAVSP YQQVIEKTKS LSFRSQMLAM 420
NIEKKLNQNS RSEAPNWATQ DSGFY 445 |
Gene Ontology | GO:0016282; C:eukaryotic 43S preinitiation complex; IEA:HAMAP. GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:HAMAP. GO:0005852; C:eukaryotic translation initiation factor 3 complex; ISS:UniProtKB. GO:0016605; C:PML body; IEA:UniProtKB-SubCell. GO:0003743; F:translation initiation factor activity; IEA:HAMAP. GO:0001731; P:formation of translation preinitiation complex; IEA:HAMAP. GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; ISS:UniProtKB. GO:0006446; P:regulation of translational initiation; IEA:HAMAP. GO:0006413; P:translational initiation; ISS:UniProtKB. |
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