UniProt Accession

 SF3A2_HUMAN; Q15428; B2RBU1; D6W605; O75245 

Genbank Protein ID

 L21990; AK314815; AC005263; CH471139; CH471139; CH471139; CH471139; BC004434; BC009903 

Genbank Nucleotide ID

 AAA60301.1; BAG37338.1; AAC25613.1; EAW69398.1; EAW69400.1; EAW69401.1; EAW69402.1; AAH04434.1; AAH09903.1 

Protein Name

 Splicing factor 3A subunit 2 

Protein Synonyms/Alias

 SF3a66; Spliceosome-associated protein 62; SAP 62 

Gene Name

 SF3A2 

Gene Synonyms/Alias

 SAP62 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
10	FQHRPGGKTGSGGVA	ubiquitination	[1]
42	LETIDINKDPYFMKN	ubiquitination	[1, 2]
48	NKDPYFMKNHLGSYE	ubiquitination	[1, 3]
91	RRAAKEAKEAPAQPA	ubiquitination	[1]
111	VEVKKFVKIGRPGYK	ubiquitination	[1]
118	KIGRPGYKVTKQRDS	ubiquitination	[1]
194	EIDKAEGKFWTHWNR	ubiquitination	[3]
216	QFHFKMEKPPAPPSL	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 

Functional Description

 Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. 

Sequence Annotation

 ZN_FING 54 84 Matrin-type.
 MOD_RES 1 1 N-acetylmethionine.  

Keyword

 Acetylation; Complete proteome; Direct protein sequencing; Metal-binding; mRNA processing; mRNA splicing; Nucleus; Reference proteome; Repeat; Spliceosome; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 464 AA 

Protein Sequence

Gene Ontology

 GO:0071013; C:catalytic step 2 spliceosome; IDA:UniProtKB.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0030532; C:small nuclear ribonucleoprotein complex; TAS:ProtInc.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0000389; P:mRNA 3'-splice site recognition; TAS:HGNC. 

Interpro

 IPR019134; Cactin_C.
 IPR015880; Znf_C2H2-like.
 IPR000690; Znf_C2H2_matrin.
 IPR003604; Znf_U1. 

Pfam

  

SMART

 SM01050; CactinC_cactus
 SM00355; ZnF_C2H2
 SM00451; ZnF_U1 

PROSITE

 PS50171; ZF_MATRIN 

PRINTS